The Release of Lactate Dehydrogenase from Chick Embryo Cells Infected with Semliki Forest Virus
| العنوان: | The Release of Lactate Dehydrogenase from Chick Embryo Cells Infected with Semliki Forest Virus |
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| المؤلفون: | A. C. Cassells |
| المصدر: | Journal of General Virology. 18:203-205 |
| بيانات النشر: | Microbiology Society, 1973. |
| سنة النشر: | 1973 |
| مصطلحات موضوعية: | Time Factors, viruses, Hemagglutinins, Viral, Chick Embryo, Tritium, Virus Replication, Semliki Forest virus, Virus, chemistry.chemical_compound, Virology, Lactate dehydrogenase, Animals, Uridine, Cells, Cultured, chemistry.chemical_classification, Growth medium, L-Lactate Dehydrogenase, biology, virus diseases, Embryo, Ldh release, biology.organism_classification, Semliki forest virus, Molecular biology, Culture Media, Enzyme, chemistry, Time course, Dactinomycin, RNA, Viral |
| الوصف: | During the course of an investigation into changes in host-coded enzymes of chick embryo cells (CEC) following infection with Semliki Forest virus (SFV) (Cassells & Burke, 1973) it was observed that, after an initial increase, there was a decrease in the activity of cellular lactate dehydrogenase (LDH: EC 1.1.1.27) which coincided with an increase in LDH activity in the growth medium. Further investigation showed that the release of LDH by the cells was an indicator of SFV growth. It was also found that treatment of CE cells with actinomycin D (AMD) resulted in LDH release. The time course of LDH release is compared in Fig. 1 with other indicators of SFV growth, namely virus RNA synthesis as measured by [3H]-uridine incorporation, virus haemagglutinin production and the release of infective virus. |
| تدمد: | 1465-2099 0022-1317 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2580e705b536058632be947fc4d5018dTest https://doi.org/10.1099/0022-1317-18-2-203Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2580e705b536058632be947fc4d5018d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14652099 00221317 |
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