Permissive and restricted virus infection of murine embryonic stem cells
| العنوان: | Permissive and restricted virus infection of murine embryonic stem cells |
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| المؤلفون: | Rachael S. Wash, David Goulding, Stacey Efstathiou, E-Pien Tan, Sabrina Calabressi, Jürgen Haas, Paul Digard, Samantha J. Griffiths, Helen M. Wise, Stephanie Franz, Paul Kellam |
| المصدر: | Wash, R, Calabressi, S, Franz, S, Griffiths, S J, Goulding, D, Tan, E-P, Wise, H, Digard, P, Haas, J, Efstathiou, S & Kellam, P 2012, ' Permissive and Restricted Virus Infection of Murine Embryonic Stem Cells ', Journal of General Virology, vol. 93, no. 10, pp. 2118-2130 . https://doi.org/10.1099/vir.0.043406-0Test The Journal of General Virology |
| بيانات النشر: | Microbiology Society, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | DNA Replication, Cell type, animal structures, Transcription, Genetic, viruses, Herpesvirus 1, Human, Biology, Virus Replication, Virus, Cell Line, Mice, Viral Proteins, 03 medical and health sciences, Dogs, 0302 clinical medicine, Orthomyxoviridae Infections, RNA interference, Cricetinae, Virology, Animals, Humans, RNA, Small Interfering, Embryonic Stem Cells, 030304 developmental biology, Mice, Knockout, 0303 health sciences, Innate immune system, Animal, Herpes Simplex, 11 Medical And Health Sciences, 06 Biological Sciences, Embryonic stem cell, Standard, 3. Good health, Mice, Inbred C57BL, Viral replication, Influenza A virus, Cell culture, Host-Pathogen Interactions, 07 Agricultural And Veterinary Sciences, Stem cell, DNA viruses, 030217 neurology & neurosurgery, HeLa Cells |
| الوصف: | Recent RNA interference (RNAi) studies have identified many host proteins that modulate virus infection, but small interfering RNA ‘off-target’ effects and the use of transformed cell lines limit their conclusiveness. As murine embryonic stem (mES) cells can be genetically modified and resources exist where many and eventually all known mouse genes are insertionally inactivated, it was reasoned that mES cells would provide a useful alternative to RNAi screens. Beyond allowing investigation of host–pathogen interactions in vitro, mES cells have the potential to differentiate into other primary cell types, as well as being used to generate knockout mice for in vivo studies. However, mES cells are poorly characterized for virus infection. To investigate whether ES cells can be used to explore host–virus interactions, this study characterized the responses of mES cells following infection by herpes simplex virus type 1 (HSV-1) and influenza A virus. HSV-1 replicated lytically in mES cells, although mES cells were less permissive than most other cell types tested. Influenza virus was able to enter mES cells and express some viral proteins, but the replication cycle was incomplete and no infectious virus was produced. Knockdown of the host protein AHCYL1 in mES cells reduced HSV-1 replication, showing the potential for using mES cells to study host–virus interactions. Transcriptional profiling, however, indicated the lack of an efficient innate immune response in these cells. mES cells may thus be useful to identify host proteins that play a role in virus replication, but they are not suitable to determine factors that are involved in innate host defence. |
| وصف الملف: | application/pdf |
| تدمد: | 1465-2099 0022-1317 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f984579056c8abd2bfa363f26b74276aTest https://doi.org/10.1099/vir.0.043406-0Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f984579056c8abd2bfa363f26b74276a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14652099 00221317 |
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