تقرير
Orchestration of the stilbene synthase gene family and their regulators by subgroup 2 MYB genes
| العنوان: | Orchestration of the stilbene synthase gene family and their regulators by subgroup 2 MYB genes |
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| المؤلفون: | Orduña, Luis, Li, Miaomiao, Navarro-Payá, David, Zhang, Chen, Ramšak, Živa, Gruden, Kristina, Höll, Janine, Merz, Patrick, Vannozzi, Alessandro, Cantu, Dario, Bogs, Jochen, Wong, Darren C. J., Huang, Shao-shan Carol, Matus, José Tomás |
| المساهمون: | Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, China Scholarship Council |
| بيانات النشر: | MedRxiv |
| سنة النشر: | 2021 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| الوصف: | The control of plant specialised metabolism is exerted by transcription factors and co-regulators acting on cis-regulatory DNA sequences of pathway-structural genes, determining when, where, and how metabolites are accumulated. A particularly interesting case for studying the transcriptional control of metabolism is represented by stilbenoids, produced within the phenylpropanoid pathway, as their ability to inhibit infection by coronaviruses MERS-CoV and SARS-CoV has been recently demonstrated in vitro. Integrative omic studies in grapevine (Vitis vinifera L.), including gene co-expression networks, have previously highlighted several transcription factors (TFs) from different gene families as potential modulators of stilbenoid accumulation, offering an ideal framework for gene function characterisation using genome-wide approaches. In the context of non-model plant species, DNA affinity purification sequencing (DAP-Seq) results a novel and potentially powerful tool for the analysis of novel uncharacterised regulators, however, it has not yet been applied in fruit crops. Accordingly, we tested as a proof-of-concept the binding of two previously characterised R2R3-MYB TFs to their known targets of the stilbene pathway, MYB14 and MYB15, obtaining 5,222 and 4,502 binding events assigned to 4,038 and 3,645 genes for each TF, respectively. Bound genes (putative targets) were overlapped with aggregated gene centred co-expression networks resulting in shared and exclusive High Confidence Targets (HCTs) suggesting a high, but not complete, redundancy. Our results show that in addition to the previously known but few STS targets, these regulators bind to almost half of the complete STS family in addition to other phenylpropanoid- and stilbenoid-related genes. We also suggest they are potentially involved in other processes such as the circadian rhythm or the synthesis of biotin. We searched the activated transcriptomes of transiently MYB15-overexpressing grapevine plants and observed a large activation of its high ... |
| نوع الوثيقة: | report |
| اللغة: | English |
| العلاقة: | #PLACEHOLDER_PARENT_METADATA_VALUE#; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-099449-A-I00; PGC2018-099449-A-I00/AEI/10.13039/501100011033; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RYC-2017-23645; RYC-2017-23645/AEI/10.13039/501100011033; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PRE2019-088044; PRE2019-088044/AEI/10.13039/501100011033; Preprint; https://doi.org/10.1101/2020.12.31.424746Test; Sí; MedRxiv: 10.1101/2020.12.31.424746 (2020); http://hdl.handle.net/10261/226747Test; http://dx.doi.org/10.13039/501100000780Test; http://dx.doi.org/10.13039/501100004543Test; http://dx.doi.org/10.13039/501100011033Test |
| DOI: | 10.1101/2020.12.31.424746 |
| DOI: | 10.13039/501100000780 |
| DOI: | 10.13039/501100004543 |
| DOI: | 10.13039/501100011033 |
| الإتاحة: | https://doi.org/10.1101/2020.12.31.424746Test https://doi.org/10.13039/501100000780Test https://doi.org/10.13039/501100004543Test https://doi.org/10.13039/501100011033Test http://hdl.handle.net/10261/226747Test |
| حقوق: | open |
| رقم الانضمام: | edsbas.3E5C574B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1101/2020.12.31.424746 |
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