Proprotein convertase 1/3-mediated down-regulation of brain-derived neurotrophic factor in cortical neurons induced by oxygen-glucose deprivation
| العنوان: | Proprotein convertase 1/3-mediated down-regulation of brain-derived neurotrophic factor in cortical neurons induced by oxygen-glucose deprivation |
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| المؤلفون: | Feng Liu, Zhaohui Zhang, Yan Chen, Xiang-Yang Zhang, Wei-Chun Guo |
| المصدر: | Neural Regeneration Research, Vol 15, Iss 6, Pp 1066-1070 (2020) Neural Regeneration Research |
| بيانات النشر: | Medknow, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, oxygen-glucose deprivation, endocrine system, medicine.medical_specialty, proprotein convertase 1/3, precursor protein of brain-derived neurotrophic factor, Synaptogenesis, Proprotein convertase 1, ischemia, Neurotransmission, lcsh:RC346-429, 03 medical and health sciences, 0302 clinical medicine, Developmental Neuroscience, Neurotrophic factors, Internal medicine, medicine, lcsh:Neurology. Diseases of the nervous system, Brain-derived neurotrophic factor, biology, neurotrophin, cortical neuron, Proprotein convertase, 030104 developmental biology, Endocrinology, nervous system, biology.protein, proprotein convertase, 030217 neurology & neurosurgery, Intracellular, Research Article, Neurotrophin |
| الوصف: | Brain-derived neurotrophic factor (BDNF) has robust effects on synaptogenesis, neuronal differentiation and synaptic transmission and plasticity. The maturation of BDNF is a complex process. Proprotein convertase 1/3 (PC1/3) has a key role in the cleavage of protein precursors that are directed to regulated secretory pathways; however, it is not clear whether PC1/3 mediates the change in BDNF levels caused by ischemia. To clarify the role of PC1/3 in BDNF maturation in ischemic cortical neurons, primary cortical neurons from fetal rats were cultured in a humidified environment of 95% N2 and 5% CO2 in a glucose-free Dulbecco’s modified Eagle’s medium at 37°C for 3 hours. Enzyme-linked immunosorbent assays and western blotting showed that after oxygen-glucose deprivation, the secreted and intracellular levels of BDNF were significantly reduced and the intracellular level of PC1/3 was decreased. Transient transfection of cortical neurons with a PC1/3 overexpression plasmid followed by oxygen-glucose deprivation resulted in increased PC1/3 levels and increased BDNF levels. When levels of the BDNF precursor protein were reduced, the concentration of BDNF in the culture medium was increased. These results indicate that PC1/3 cleavage of BDNF is critical for the conversion of pro-BDNF in rat cortical neurons during ischemia. The study was approved by the Animal Ethics Committee of Wuhan University School of Basic Medical Sciences. |
| تدمد: | 1673-5374 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12a891e6befcc2f7fe538ff93427b1f4Test https://doi.org/10.4103/1673-5374.270314Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....12a891e6befcc2f7fe538ff93427b1f4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16735374 |
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