Crescent-Like Lesions as an Early Signature of Nephropathy in a Rat Model of Prediabetes Induced by a Hypercaloric Diet
| العنوان: | Crescent-Like Lesions as an Early Signature of Nephropathy in a Rat Model of Prediabetes Induced by a Hypercaloric Diet |
|---|---|
| المؤلفون: | Pedro Vieira, Flávio Reis, Sofia D. Viana, Sara Nunes, Inês Preguiça, Fernando Mendes, Adelaide Barbosa, André Alves, Diana Raquel Fernandes Martins |
| المساهمون: | Instituto de Investigação e Inovação em Saúde |
| المصدر: | Nutrients, Vol 12, Iss 4, p 881 (2020) Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) Agência para a Sociedade do Conhecimento (UMIC)-FCT-Sociedade da Informação instacron:RCAAP Nutrients |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, Kidney Glomerulus, prediabetes, 030204 cardiovascular system & hematology, Kidney Glomerulus / metabolism, Kidney Function Tests, diet-induced, Prediabetic State / complications, Diabetic nephropathy, Impaired glucose tolerance, Diabetic Nephropathies / etiology, 0302 clinical medicine, Diet, Diabetic, Diabetic Nephropathies, Prediabetes, Diabetic Nephropathies / metabolism, Nutrition and Dietetics, Communication, Immunohistochemistry, nephropathy, lcsh:Nutrition. Foods and food supply, Collagen Type IV, medicine.medical_specialty, Kidney Glomerulus / pathology, Renal function, 030209 endocrinology & metabolism, lcsh:TX341-641, Diabetic Nephropathies / pathology, Prediabetic State / metabolismo, Nephropathy, Prediabetic State, 03 medical and health sciences, Diet, Diabetic / adverse effects, Internal medicine, Diabetes mellitus, Collagen Type IV / metabolism, medicine, Animals, business.industry, rat model, Hypertriglyceridemia, Body Weight, Prediabetic State / etiology, glomerular crescent-like lesions, medicine.disease, Lipid Metabolism, Rats, Disease Models, Animal, Endocrinology, renal lipidosis, business, Energy Intake, Biomarkers, Food Science, Kidney disease |
| الوصف: | Diabetic nephropathy (DN) is a major microvascular complication of diabetes. Obesity and hyperlipidemia, fueled by unhealthy food habits, are risk factors to glomerular filtration rate (GFR) decline and DN progression. Several studies recommend that diabetic patients should be screened early (in prediabetes) for kidney disease, in order to prevent advanced stages, for whom the current interventions are clearly inefficient. This ambition greatly depends on the existence of accurate early biomarkers and novel molecular targets, which only may arise with a more thorough knowledge of disease pathophysiology. We used a rat model of prediabetes induced by 23 weeks of high-sugar/high-fat (HSuHF) diet to characterize the phenotype of early renal dysfunction and injury. When compared with the control animals, HSuHF-treated rats displayed a metabolic phenotype compatible with obese prediabetes, displaying impaired glucose tolerance and insulin sensitivity, along with hypertriglyceridemia, and lipid peroxidation. Despite unchanged creatinine levels, the prediabetic animals presented glomerular crescent-like lesions, accompanied by increased kidney Oil-Red-O staining, triglycerides content and mRNA expression of IL-6 and iNOS. This model of HSuHF-induced prediabetes can be a useful tool to study early features of DN, namely crescent-like lesions, an early signature that deserves in-depth elucidation. This work was supported by the European Regional Development Fund (FEDER), through Programa Operacional Factores de Competitividade COMPETE2020 (CENTRO-01-0145-FEDER-000012-HealthyAging2020) and by National funds via Portuguese Science and Technology Foundation (FCT): Strategic Projects UID/NEU/04539/2013, UID/NEU/04539/2019, UIDB/04539/2020 and UIDP/04539/2020 (CIBB), SFRH/BD/109017/2015 (PhD Fellowship) and PTDC/SAU-NUT/31712/2017, as well as by COMPETE-FEDER funds (POCI-01-0145-FEDER-007440 and POCI-01-0145-FEDER-031712). |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2072-6643 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::589f17502d2721c822726c59205ab205Test https://www.mdpi.com/2072-6643/12/4/881Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....589f17502d2721c822726c59205ab205 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20726643 |
|---|