دورية أكاديمية
A Tripartite Efflux System Affects Flagellum Stability in Helicobacter pylori
العنوان: | A Tripartite Efflux System Affects Flagellum Stability in Helicobacter pylori |
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المؤلفون: | Katherine Gibson, Joshua K. Chu, Shiwei Zhu, Doreen Nguyen, Jan Mrázek, Jun Liu, Timothy R. Hoover |
المصدر: | International Journal of Molecular Sciences, Vol 23, Iss 19, p 11609 (2022) |
بيانات النشر: | MDPI AG, 2022. |
سنة النشر: | 2022 |
المجموعة: | LCC:Biology (General) LCC:Chemistry |
مصطلحات موضوعية: | Helicobacter pylori, flagellum, flagellar motor, flagellar sheath, Biology (General), QH301-705.5, Chemistry, QD1-999 |
الوصف: | Helicobacter pylori uses a cluster of polar, sheathed flagella for swimming motility. A search for homologs of H. pylori proteins that were conserved in Helicobacter species that possess flagellar sheaths but were underrepresented in Helicobacter species with unsheathed flagella identified several candidate proteins. Four of the identified proteins are predicted to form part of a tripartite efflux system that includes two transmembrane domains of an ABC transporter (HP1487 and HP1486), a periplasmic membrane fusion protein (HP1488), and a TolC-like outer membrane efflux protein (HP1489). Deleting hp1486/hp1487 and hp1489 homologs in H. pylori B128 resulted in reductions in motility and the number of flagella per cell. Cryo-electron tomography studies of intact motors of the Δhp1489 and Δhp1486/hp1487 mutants revealed many of the cells contained a potential flagellum disassembly product consisting of decorated L and P rings, which has been reported in other bacteria. Aberrant motors lacking specific components, including a cage-like structure that surrounds the motor, were also observed in the Δhp1489 mutant. These findings suggest a role for the H. pylori HP1486-HP1489 tripartite efflux system in flagellum stability. Three independent variants of the Δhp1486/hp1487 mutant with enhanced motility were isolated. All three motile variants had the same frameshift mutation in fliL, suggesting a role for FliL in flagellum disassembly. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1422-0067 1661-6596 |
العلاقة: | https://www.mdpi.com/1422-0067/23/19/11609Test; https://doaj.org/toc/1661-6596Test; https://doaj.org/toc/1422-0067Test |
DOI: | 10.3390/ijms231911609 |
الوصول الحر: | https://doaj.org/article/edbb99c92bb0466ca8f0addad9c11ac5Test |
رقم الانضمام: | edsdoj.bb99c92bb0466ca8f0addad9c11ac5 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 14220067 16616596 |
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DOI: | 10.3390/ijms231911609 |