دورية أكاديمية
Multiple Nucleocapsid Structural Forms of Shrimp White Spot Syndrome Virus Suggests a Novel Viral Morphogenetic Pathway
| العنوان: | Multiple Nucleocapsid Structural Forms of Shrimp White Spot Syndrome Virus Suggests a Novel Viral Morphogenetic Pathway |
|---|---|
| المؤلفون: | Hui-Ju Huang, Sen-Lin Tang, Yuan-Chih Chang, Hao-Ching Wang, Tze Hann Ng, Rees F. Garmann, Yu-Wen Chen, Jiun-Yan Huang, Ramya Kumar, Sheng-Hsiung Chang, Shang-Rung Wu, Chih-Yu Chao, Kyoko Matoba, Iwasaki Kenji, William M. Gelbart, Tzu-Ping Ko, Hwei-Jiung (Andrew) Wang, Chu-Fang Lo, Li-Li Chen, Han-Ching Wang |
| المصدر: | International Journal of Molecular Sciences, Vol 24, Iss 8, p 7525 (2023) |
| بيانات النشر: | MDPI AG |
| سنة النشر: | 2023 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | white spot syndrome virus, large dsDNA virus, cryogenic electron microscopy, nucleocapsid, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | White spot syndrome virus (WSSV) is a very large dsDNA virus. The accepted shape of the WSSV virion has been as ellipsoidal, with a tail-like extension. However, due to the scarcity of reliable references, the pathogenesis and morphogenesis of WSSV are not well understood. Here, we used transmission electron microscopy (TEM) and cryogenic electron microscopy (Cryo-EM) to address some knowledge gaps. We concluded that mature WSSV virions with a stout oval-like shape do not have tail-like extensions. Furthermore, there were two distinct ends in WSSV nucleocapsids: a portal cap and a closed base. A C14 symmetric structure of the WSSV nucleocapsid was also proposed, according to our Cryo-EM map. Immunoelectron microscopy (IEM) revealed that VP664 proteins, the main components of the 14 assembly units, form a ring-like architecture. Moreover, WSSV nucleocapsids were also observed to undergo unique helical dissociation. Based on these new results, we propose a novel morphogenetic pathway of WSSV. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| العلاقة: | https://www.mdpi.com/1422-0067/24/8/7525Test; https://doaj.org/toc/1661-6596Test; https://doaj.org/toc/1422-0067Test; https://doaj.org/article/1ab719f065a24a2aa833f53af96ca3b2Test |
| DOI: | 10.3390/ijms24087525 |
| الإتاحة: | https://doi.org/10.3390/ijms24087525Test https://doaj.org/article/1ab719f065a24a2aa833f53af96ca3b2Test |
| رقم الانضمام: | edsbas.6E39CB44 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14220067 16616596 |
|---|---|
| DOI: | 10.3390/ijms24087525 |