Genetic Diversity of Babesia bovis MSA-1, MSA-2b and MSA-2c in China
| العنوان: | Genetic Diversity of Babesia bovis MSA-1, MSA-2b and MSA-2c in China |
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| المؤلفون: | Guiquan Guan, Jinming Wang, Jifei Yang, Xiaoxing Wang, Hao Sun, Hong Yin, Zhaoyong Lv, Youquan Li, Junlong Liu, Jianxun Luo, Aihong Liu, Shandian Gao |
| المصدر: | Pathogens, Vol 9, Iss 473, p 473 (2020) Pathogens Volume 9 Issue 6 |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Microbiology (medical), Genetics, Genetic diversity, China, General Immunology and Microbiology, biology, Phylogenetic tree, lcsh:R, lcsh:Medicine, Babesia bovis, genetic diversity, biology.organism_classification, law.invention, Infectious Diseases, Antigen, law, parasitic diseases, Immunology and Allergy, Parasite hosting, merozoite surface antigen, Molecular Biology, Nested polymerase chain reaction, Gene, Polymerase chain reaction |
| الوصف: | The apicomplexan parasite Babesia bovis is a tick-borne intracellular hemoprotozoan parasite that is widespread across China. Genetic diversity is an important strategy used by parasites to escape the immune responses of their hosts. In our present study, 575 blood samples, collected from cattle in 10 provinces, were initially screened using a nested PCR (polymerase chain reaction) for detection of B. bovis infection. To perform genetic diversity analyses, positive samples were further amplified to obtain sequences of three B. bovis merozoite surface antigen genes (MSA-1, MSA-2b, MSA-2c). The results of the nested PCR approach showed that an average of 8.9% (51/575) of cattle were positive for B. bovis infection. Phylogenetic analyses of the predicted amino acid sequences revealed that unique antigen variants were formed only by Chinese isolates. Our findings provide vital information for understanding the genetic diversity of B. bovis in China. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2076-0817 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54edd07498bdb9f330aa21a41f0b9f43Test https://www.mdpi.com/2076-0817/9/6/473Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....54edd07498bdb9f330aa21a41f0b9f43 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20760817 |
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