Alpha-Lipoic Acid Plays a Role in Endometriosis: New Evidence on Inflammasome-Mediated Interleukin Production, Cellular Adhesion and Invasion
| العنوان: | Alpha-Lipoic Acid Plays a Role in Endometriosis: New Evidence on Inflammasome-Mediated Interleukin Production, Cellular Adhesion and Invasion |
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| المؤلفون: | Roberta Castellani, Carmela Paciullo, Alessandra De Cicco Nardone, Alfredo Pontecorvi, Fiorella Di Nicuolo, Giovanni Scambia, Nicoletta Di Simone, Greta Barbaro |
| المصدر: | Molecules, Vol 26, Iss 288, p 288 (2021) Molecules Volume 26 Issue 2 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | endometriosis, Cell signaling, Stromal cell, Interleukin-1beta, Pharmaceutical Science, Matrix metalloproteinase, estrogen receptor (ER), NALP-3 inflammasome, Article, Cell Line, Analytical Chemistry, lcsh:QD241-441, Endometrium, 03 medical and health sciences, 0302 clinical medicine, lcsh:Organic chemistry, NLR Family, Pyrin Domain-Containing 3 Protein, Drug Discovery, Cell Adhesion, medicine, Estrogen Receptor beta, Humans, alpha-lipoic acid, estrogen receptor (ER) β, Physical and Theoretical Chemistry, Cell adhesion, 030304 developmental biology, 0303 health sciences, α-lipoic acid, 030219 obstetrics & reproductive medicine, Thioctic Acid, Chemistry, Cell adhesion molecule, Organic Chemistry, Interleukin-18, Interleukin, Settore MED/13 - ENDOCRINOLOGIA, Inflammasome, Settore MED/40 - GINECOLOGIA E OSTETRICIA, Chemistry (miscellaneous), Cancer research, Molecular Medicine, Female, Signal transduction, hormones, hormone substitutes, and hormone antagonists, Signal Transduction, medicine.drug |
| الوصف: | Endometriosis is an estrogen-linked gynecological disease defined by the presence of endometrial tissue on extrauterine sites where it forms invasive lesions. Alterations in estrogen-mediated cellular signaling seems to have an essential role in the pathogenesis of endometriosis. Higher estrogen receptor (ER)-&beta levels and enhanced ER-&beta activity were detected in endometriotic tissues. It is well known that ER-&beta interacts with components of the cytoplasmic inflammasome-3 (NALP-3), the NALP-3 activation increases interleukin (IL)-1&beta and IL-18, enhancing cellular adhesion and proliferation. Otherwise, the inhibition of ER-&beta activity suppresses the ectopic lesions growth. The present study aims to investigate the potential effect of &alpha lipoic acid (ALA) on NALP-3 and ER-&beta expression using a western blot analysis, NALP-3-induced cytokines production by ELISA, migration and invasion of immortalized epithelial (12Z) and stromal endometriotic cells (22B) using a 3D culture invasion assay, and matrix-metalloprotease (MMPs) activity using gelatin zymography. ALA significantly reduces ER-&beta NALP-3 protein expression/activity and the secretion of IL-1&beta and IL-18 in both 12Z and 22B cells. ALA treatment reduces cellular adhesion and invasion via a lower expression of adhesion molecules and MMPs activities. These results provide convincing evidence that ALA might inhibit endometriosis progression. |
| وصف الملف: | application/pdf |
| تدمد: | 1420-3049 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f173bada59de9512248f6d1bc0552c39Test https://doi.org/10.3390/molecules26020288Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f173bada59de9512248f6d1bc0552c39 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14203049 |
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