دورية أكاديمية
Increased Chromosome Aberrations in Cells Exposed Simultaneously to Simulated Microgravity and Radiation
| العنوان: | Increased Chromosome Aberrations in Cells Exposed Simultaneously to Simulated Microgravity and Radiation |
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| المؤلفون: | Megumi Hada, Hiroko Ikeda, Jordan R. Rhone, Andrew J. Beitman, Ianik Plante, Hikaru Souda, Yukari Yoshida, Kathryn D. Held, Keigi Fujiwara, Premkumar B. Saganti, Akihisa Takahashi |
| المصدر: | International Journal of Molecular Sciences, Vol 20, Iss 1, p 43 (2018) |
| بيانات النشر: | MDPI AG, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | microgravity, ionizing radiation, space radiation, chromosome aberration, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Space radiation and microgravity (μG) are two major environmental stressors for humans in space travel. One of the fundamental questions in space biology research is whether the combined effects of μG and exposure to cosmic radiation are interactive. While studies addressing this question have been carried out for half a century in space or using simulated μG on the ground, the reported results are ambiguous. For the assessment and management of human health risks in future Moon and Mars missions, it is necessary to obtain more basic data on the molecular and cellular responses to the combined effects of radiation and µG. Recently we incorporated a μG–irradiation system consisting of a 3D clinostat synchronized to a carbon-ion or X-ray irradiation system. Our new experimental setup allows us to avoid stopping clinostat rotation during irradiation, which was required in all other previous experiments. Using this system, human fibroblasts were exposed to X-rays or carbon ions under the simulated μG condition, and chromosomes were collected with the premature chromosome condensation method in the first mitosis. Chromosome aberrations (CA) were quantified by the 3-color fluorescent in situ hybridization (FISH) method. Cells exposed to irradiation under the simulated μG condition showed a higher frequency of both simple and complex types of CA compared to cells irradiated under the static condition by either X-rays or carbon ions. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 |
| العلاقة: | http://www.mdpi.com/1422-0067/20/1/43Test; https://doaj.org/toc/1422-0067Test |
| DOI: | 10.3390/ijms20010043 |
| الوصول الحر: | https://doaj.org/article/5e4599c9110c41af8f74696389fad61fTest |
| رقم الانضمام: | edsdoj.5e4599c9110c41af8f74696389fad61f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 |
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| DOI: | 10.3390/ijms20010043 |