Multiple Nucleocapsid Structural Forms of Shrimp White Spot Syndrome Virus Suggests a Novel Viral Morphogenetic Pathway

التفاصيل البيبلوغرافية
العنوان: Multiple Nucleocapsid Structural Forms of Shrimp White Spot Syndrome Virus Suggests a Novel Viral Morphogenetic Pathway
المؤلفون: Hui-Ju Huang, Sen-Lin Tang, Yuan-Chih Chang, Hao-Ching Wang, Tze Hann Ng, Rees F. Garmann, Yu-Wen Chen, Jiun-Yan Huang, Ramya Kumar, Sheng-Hsiung Chang, Shang-Rung Wu, Chih-Yu Chao, Kyoko Matoba, Iwasaki Kenji, William M. Gelbart, Tzu-Ping Ko, Hwei-Jiung (Andrew) Wang, Chu-Fang Lo, Li-Li Chen, Han-Ching Wang
المصدر: International Journal of Molecular Sciences; Volume 24; Issue 8; Pages: 7525
بيانات النشر: MDPI AG, 2023.
سنة النشر: 2023
مصطلحات موضوعية: Inorganic Chemistry, Organic Chemistry, white spot syndrome virus, large dsDNA virus, cryogenic electron microscopy, nucleocapsid, General Medicine, Physical and Theoretical Chemistry, Molecular Biology, Spectroscopy, Catalysis, Computer Science Applications
الوصف: White spot syndrome virus (WSSV) is a very large dsDNA virus. The accepted shape of the WSSV virion has been as ellipsoidal, with a tail-like extension. However, due to the scarcity of reliable references, the pathogenesis and morphogenesis of WSSV are not well understood. Here, we used transmission electron microscopy (TEM) and cryogenic electron microscopy (Cryo-EM) to address some knowledge gaps. We concluded that mature WSSV virions with a stout oval-like shape do not have tail-like extensions. Furthermore, there were two distinct ends in WSSV nucleocapsids: a portal cap and a closed base. A C14 symmetric structure of the WSSV nucleocapsid was also proposed, according to our Cryo-EM map. Immunoelectron microscopy (IEM) revealed that VP664 proteins, the main components of the 14 assembly units, form a ring-like architecture. Moreover, WSSV nucleocapsids were also observed to undergo unique helical dissociation. Based on these new results, we propose a novel morphogenetic pathway of WSSV.
وصف الملف: application/pdf
تدمد: 1422-0067
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f36d436011d178ca68590158d80cc2adTest
https://doi.org/10.3390/ijms24087525Test
حقوق: OPEN
رقم الانضمام: edsair.doi.dedup.....f36d436011d178ca68590158d80cc2ad
قاعدة البيانات: OpenAIRE