Oxidative Stress and Analysis of Selected SNPs of ACHE (rs 2571598), BCHE (rs 3495), CAT (rs 7943316), SIRT1 (rs 10823108), GSTP1 (rs 1695), and Gene GSTM1, GSTT1 in Chronic Organophosphates Exposed Groups from Cameroon and Pakistan
| العنوان: | Oxidative Stress and Analysis of Selected SNPs of ACHE (rs 2571598), BCHE (rs 3495), CAT (rs 7943316), SIRT1 (rs 10823108), GSTP1 (rs 1695), and Gene GSTM1, GSTT1 in Chronic Organophosphates Exposed Groups from Cameroon and Pakistan |
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| المؤلفون: | Syed M. Nurulain, Rabia Habib, Leonel Javeres Mbah Ntepe, Sajida Batool, Ngondi Judith Laure, Eugenie Nepovimova, Saqlain Raza, Kamil Kuca |
| المصدر: | International Journal of Molecular Sciences Volume 21 Issue 17 |
| بيانات النشر: | MDPI, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, 010501 environmental sciences, Pharmacology, medicine.disease_cause, 01 natural sciences, chemistry.chemical_compound, GSTP1, 0302 clinical medicine, Sirtuin 1, Malondialdehyde, Pakistan, Cameroon, cholinergic enzymes, Spectroscopy, Glutathione Transferase, biology, General Medicine, Middle Aged, toxicogenetics, Catalase, Acetylcholinesterase, Glutathione, Computer Science Applications, antioxidants, Female, SNPs, Adult, Adolescent, GPI-Linked Proteins, Polymorphism, Single Nucleotide, Catalysis, Article, Inorganic Chemistry, Superoxide dismutase, 03 medical and health sciences, Young Adult, Organophosphorus Compounds, organophosphates, medicine, Humans, Physical and Theoretical Chemistry, Molecular Biology, 0105 earth and related environmental sciences, Cholinesterase, Organic Chemistry, Environmental Exposure, Oxidative Stress, chemistry, Glutathione S-Transferase pi, Butyrylcholinesterase, biology.protein, Gene-Environment Interaction, 030217 neurology & neurosurgery, Oxidative stress |
| الوصف: | The detrimental effects of organophosphates (OPs) on human health are thought to be of systemic, i.e., irreversible inhibition of acetylcholinesterase (AChE) at nerve synapses. However, several studies have shown that AChE inhibition alone cannot explain all the toxicological manifestations in prolonged exposure to OPs. The present study aimed to assess the status of antioxidants malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) (reduced), catalase, and ferric reducing antioxidant power (FRAP) in chronic OP-exposed groups from Cameroon and Pakistan. Molecular analysis of genetic polymorphisms (SNPs) of glutathione transferases (GSTM1, GSTP1, GSTT1), catalase gene (CAT, rs7943316), sirtuin 1 gene (SIRT1, rs10823108), acetylcholinesterase gene (ACHE, rs2571598), and butyrylcholinesterase gene (BCHE, rs3495) were screened in the OP-exposed individuals to find the possible causative association with oxidative stress and toxicity. Cholinesterase and antioxidant activities were measured by colorimetric methods using a spectrophotometer. Salting-out method was employed for DNA extraction from blood followed by restriction fragment length polymorphism (RFLP) for molecular analysis. Cholinergic enzymes were significantly decreased in OP-exposed groups. Catalase and SOD were decreased and MDA and FRAP were increased in OP-exposed groups compared to unexposed groups in both groups. GSH was decreased only in Pakistani OPs-exposed group. Molecular analysis of ACHE, BCHE, Catalase, GSTP1, and GSTM1 SNPs revealed a tentative association with their phenotypic expression that is level of antioxidant and cholinergic enzymes. The study concludes that chronic OPs exposure induces oxidative stress which is associated with the related SNP polymorphism. The toxicogenetics of understudied SNPs were examined for the first time to our understanding. The findings may lead to a newer area of investigation on OPs induced health issues and toxicogenetics. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42b949386dcb0d84220f2a9147a25999Test http://europepmc.org/articles/PMC7503738Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....42b949386dcb0d84220f2a9147a25999 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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