دورية أكاديمية
Functional Ex Vivo Testing of Alveolar Monocytes in Patients with Pneumonia-Related ARDS
| العنوان: | Functional Ex Vivo Testing of Alveolar Monocytes in Patients with Pneumonia-Related ARDS |
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| المؤلفون: | Bendib, Inès, Beldi-Ferchiou, Asma, Schlemmer, Frédéric, Maitre, Bernard, Surenaud, Mathieu, Hüe, Sophie, Carteaux, Guillaume, Razazi, Keyvan, Lelièvre, Jean-Daniel, Lévy, Yves, Mekontso Dessap, Armand, Godot, Véronique, de Prost, Nicolas |
| المساهمون: | IMRB - SMCD/"Senescence, Metabolism and Cardiovascular Diseases" Créteil (U955 Inserm - UPEC), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12) |
| المصدر: | ISSN: 2073-4409 ; Cells ; https://hal.science/hal-04392278Test ; Cells, 2021, 10 (12), pp.3546. ⟨10.3390/cells10123546⟩. |
| بيانات النشر: | HAL CCSD MDPI |
| سنة النشر: | 2021 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Biomarkers of disease severity might help with individualizing the management of patients with acute respiratory distress syndrome (ARDS). During sepsis, a sustained decreased expression of the antigen-presenting molecule human leucocyte antigen-DR (HLA-DR) on circulating monocytes is used as a surrogate marker of immune failure. This study aimed at assessing whether HLA-DR expression on alveolar monocytes in the setting of a severe lung infection is associated with their functional alterations. BAL fluid and blood from immunocompetent patients with pneumonia-related ARDS admitted between 2016 and 2018 were isolated in a prospective monocentric study. Alveolar and blood monocytes were immunophenotyped using flow cytometry. Functional tests were performed on alveolar and blood monocytes after in vitro lipopolysaccharide (LPS) stimulation. Phagocytosis activity and intracellular tumor necrosis factor (TNF) production were quantified using fluorochrome-conjugated-specific antibodies. Ten ARDS and seven non-ARDS control patients were included. Patients with pneumonia-related ARDS exhibited significantly lower HLA-DR expression both on circulating (p < 0.0001) and alveolar (p = 0.0002) monocytes. There was no statistically significant difference observed between patient groups (ARDS vs. non-ARDS) regarding both alveolar and blood monocytes phagocytosis activity. After LPS stimulation, alveolar (p = 0.027) and blood (p = 0.005) monocytes from pneumonia-related ARDS patients had a significantly lower intracellular TNF expression than non-ARDS patients. Monocytes from pneumonia-related ARDS patients have a deactivated status and an impaired TNF production capacity but display potent phagocytic activity. HLA-DR level expression should not be used as a surrogate marker of the phagocytic activity or the TNF production capacity of alveolar monocytes. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-04392278; https://hal.science/hal-04392278Test |
| DOI: | 10.3390/cells10123546 |
| الإتاحة: | https://doi.org/10.3390/cells10123546Test https://hal.science/hal-04392278Test |
| رقم الانضمام: | edsbas.CF1B8B87 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/cells10123546 |
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