التفاصيل البيبلوغرافية
| العنوان: |
scRNAseq and High-Throughput Spatial Analysis of Tumor and Normal Microenvironment in Solid Tumors Reveal a Possible Origin of Circulating Tumor Hybrid Cells. |
| المؤلفون: |
Ali, Abdullah Mahmood1,2 (AUTHOR) ama2241@cumc.columbia.edu, Raza, Azra1,2 (AUTHOR) ama2241@cumc.columbia.edu |
| المصدر: |
Cancers. Apr2024, Vol. 16 Issue 7, p1444. 20p. |
| مصطلحات موضوعية: |
*HIGH throughput screening (Drug development), *MACROPHAGES, *SURVIVAL rate, *T-test (Statistics), *TUMOR markers, *MANN Whitney U Test, *DESCRIPTIVE statistics, *RNA, *COLON tumors, *KAPLAN-Meier estimator, *GENE expression profiling, *TUMORS, *CONFIDENCE intervals, *DATA analysis software, *PHAGOCYTOSIS, *OVERALL survival |
| مستخلص: |
Simple Summary: This study explored a potentially significant player in the spread of metastatic cancer: "hybrid cells" (HCs) combining features of both epithelial (common in tissues) and immune cells (such as macrophages). HCs were found in more than one type of tumor (THC) and showed a unique transcriptional profile including the activation of functional pathways, potentially explaining their mobility, dissemination into circulation, and metastatic progression. Normal healthy tissue also showed hybrid cells (NHC), but they were rare and could be easily distinguished from THCs based on their gene expression profile. The study also developed a way to identify HCs in large datasets generated at the single-cell level, paving the way for further research on their function and as potential therapeutic targets. Metastatic cancer is a leading cause of death in cancer patients worldwide. While circulating hybrid cells (CHCs) are implicated in metastatic spread, studies documenting their tissue origin remain sparse, with limited candidate approaches using one–two markers. Utilizing high-throughput single-cell and spatial transcriptomics, we identified tumor hybrid cells (THCs) co-expressing epithelial and macrophage markers and expressing a distinct transcriptome. Rarely, normal tissue showed these cells (NHCs), but their transcriptome was easily distinguishable from THCs. THCs with unique transcriptomes were observed in breast and colon cancers, suggesting this to be a generalizable phenomenon across cancer types. This study establishes a framework for HC identification in large datasets, providing compelling evidence for their tissue residence and offering comprehensive transcriptomic characterization. Furthermore, it sheds light on their differential function and identifies pathways that could explain their newly acquired invasive capabilities. THCs should be considered as potential therapeutic targets. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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