دورية أكاديمية
NQS-Doped PDMS Solid Sensor: From Water Matrix to Urine Enzymatic Application
العنوان: | NQS-Doped PDMS Solid Sensor: From Water Matrix to Urine Enzymatic Application |
---|---|
المؤلفون: | Martínez-Aviñó, Adrià, Hakobyan, Lusine, Ballester-Caudet, Ana, Moliner-Martínez, Yolanda, Molins-Legua, Carmen, Campíns-Falcó, Pilar |
بيانات النشر: | MDPI |
سنة النشر: | 2021 |
المجموعة: | Universidad de Salamanca: Gredos (Gestión del Repositorio Documental de la Universidad de Salamanca) |
مصطلحات موضوعية: | Ammonium urea, Urease, Urea hydrolysis, NQS-PDMS sensor, Optical sensor, Glass support, In-situ analysis, Water, Urine, 2301 Química Analítica |
الوصف: | [EN] The development of in situ analytical devices has gained outstanding scientific interest. A solid sensing membrane composed of 1,2-naphthoquinone-4-sulfonate (NQS) derivatizing reagent embedded into a polymeric polydimethylsiloxane (PDMS) composite was proposed for in situ ammonium (NH4+) and urea (NH2CONH2) analysis in water and urine samples, respectively. Satisfactory strategies were also applied for urease-catalyzed hydrolysis of urea, either in solution or glass-supported urease immobilization. Using diffuse reflectance measurements combined with digital image processing of color intensity (RGB coordinates), qualitative and quantitative analyte detection was assessed after the colorimetric reaction took place inside the sensing membrane. A suitable linear relationship was found between the sensor response and analyte concentration, and the results were validated by a thymol-PDMS-based sensor based on the Berthelot reaction. The suggested sensing device offers advantages such as rapidity, versatility, portability, and employment of non-toxic reagents that facilitate in situ analysis in an energy-efficient manner. |
نوع الوثيقة: | article in journal/newspaper |
اللغة: | English |
تدمد: | 2079-6374 |
العلاقة: | https://doi.org/10.3390/bios11060186Test; http://hdl.handle.net/10366/155231Test |
الإتاحة: | https://doi.org/10.3390/bios11060186Test http://hdl.handle.net/10366/155231Test |
حقوق: | Attribution-NonCommercial-NoDerivatives 4.0 Internacional ; http://creativecommons.org/licenses/by-nc-nd/4.0Test/ ; info:eu-repo/semantics/openAccess |
رقم الانضمام: | edsbas.E2B3278A |
قاعدة البيانات: | BASE |
تدمد: | 20796374 |
---|