Isolation and Characterization of Multidrug-Resistant Escherichia coli and Salmonella spp. from Healthy and Diseased Turkeys
| العنوان: | Isolation and Characterization of Multidrug-Resistant Escherichia coli and Salmonella spp. from Healthy and Diseased Turkeys |
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| المؤلفون: | Md. Tanvir Rahman, Md. Jannat Hossain, Muhammad Tofazzal Hossain, Hossam M. Ashour, Md. Abdus Sobur, Md. Saiful Islam, Md. Muket Mahmud, Sumon Paul, Md. Tawyabur |
| المصدر: | Antibiotics Antibiotics, Vol 9, Iss 770, p 770 (2020) Volume 9 Issue 11 |
| بيانات النشر: | MDPI, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), Salmonella, salmonellosis, antibiotic resistance, Tetracycline, 030106 microbiology, Biology, medicine.disease_cause, Biochemistry, Microbiology, Article, tetA, 03 medical and health sciences, Antibiotic resistance, MDR, medicine, Pharmacology (medical), General Pharmacology, Toxicology and Pharmaceutics, Escherichia coli, lcsh:RM1-950, public health, biochemical phenomena, metabolism, and nutrition, Ciprofloxacin, Multiple drug resistance, 030104 developmental biology, Infectious Diseases, lcsh:Therapeutics. Pharmacology, Streptomycin, Gentamicin, avian colibacillosis, medicine.drug |
| الوصف: | Diseases caused by Escherichia coli (E. coli) and Salmonella spp. can negatively impact turkey farming. The aim of this study was to isolate and characterize multidrug-resistant (MDR) E. coli and Salmonella spp. in healthy and diseased turkeys. A total of 30 fecal samples from healthy turkeys and 25 intestinal samples from diseased turkeys that died of enteritis were collected. Bacterial isolation and identification were based on biochemical properties and polymerase chain reaction (PCR). Antibiogram profiles were determined by disk diffusion. The tetracycline-resistance gene tetA was detected by PCR. All samples were positive for E. coli. Only 11 samples (11/30 36.67%) were positive for Salmonella spp. from healthy turkeys, whereas 16 (16/25 64%) samples were positive for Salmonella spp. from diseased turkeys. E. coli isolated from diseased turkeys showed higher resistance to levofloxacin, gentamicin, chloramphenicol, ciprofloxacin, streptomycin, and tetracycline. Salmonella spp. isolated from healthy turkeys exhibited higher resistance to gentamicin, chloramphenicol, ciprofloxacin, streptomycin, imipenem, and meropenem. All E. coli and Salmonella spp. from both healthy and diseased turkeys were resistant to erythromycin. Salmonella spp. from both healthy and diseased turkeys were resistant to tetracycline. Multidrug resistance was observed in both E. coli and Salmonella spp. from diseased turkeys. Finally, the tetA gene was detected in 93.1% of the E. coli isolates and in 92.59% of the Salmonella spp. isolates. To the best of our knowledge, this is the first study to isolate and characterize tetA-gene-containing MDR E. coli and Salmonella spp. from healthy and diseased turkeys in Bangladesh. Both microorganisms are of zoonotic significance and represent a significant public health challenge. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2079-6382 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f360b4078a1522da23c7cad11933846fTest http://europepmc.org/articles/PMC7692380Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f360b4078a1522da23c7cad11933846f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20796382 |
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