DNA Methylation Profiles of Tph1A and BDNF in Gut and Brain of L. Rhamnosus-Treated Zebrafish
| العنوان: | DNA Methylation Profiles of Tph1A and BDNF in Gut and Brain of L. Rhamnosus-Treated Zebrafish |
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| المؤلفون: | Sergio Cocozza, Lorena Coretti, John F. Cryan, Lorenzo Chiariotti, Alessandro Fioretti, Luna D'Angelo Lancellotti di Durazzo, Giulia De Riso, Luca Borrelli, Rosa Della Monica, Mariella Cuomo, Francesca Lembo, Timothy G. Dinan |
| المساهمون: | Cuomo, M., Borrelli, L., Monica, R. D., Coretti, L., De Riso, G., Di Durazzo, L. D. L., Fioretti, A., Lembo, F., Dinan, T. G., Cryan, J. F., Cocozza, S., Chiariotti, L. |
| المصدر: | Biomolecules Biomolecules, Vol 11, Iss 142, p 142 (2021) Volume 11 Issue 2 |
| بيانات النشر: | MDPI, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, cell-to-cell heterogeneity, lcsh:QR1-502, epialleles, Tryptophan Hydroxylase, Biochemistry, lcsh:Microbiology, Epigenesis, Genetic, 0302 clinical medicine, Zebrafish, Promoter Regions, Genetic, Regulation of gene expression, Methylation profile, DNA methylation, biology, Behavior, Animal, Lacticaseibacillus rhamnosus, Microbiota, Brain, Methylation, Cell biology, Female, Danio, Context (language use), Article, 03 medical and health sciences, Animals, Epigenetics, Molecular Biology, Alleles, Gene Library, microbiota–gut–brain axis, Brain-Derived Neurotrophic Factor, Gene Expression Profiling, Probiotics, Computational Biology, Promoter, biology.organism_classification, Gastrointestinal Microbiome, Microbiota–gut–brain axi, 030104 developmental biology, Epiallele, Gene Expression Regulation, methylation profiles, CpG Islands, 030217 neurology & neurosurgery |
| الوصف: | The bidirectional microbiota&ndash gut&ndash brain axis has raised increasing interest over the past years in the context of health and disease, but there is a lack of information on molecular mechanisms underlying this connection. We hypothesized that change in microbiota composition may affect brain epigenetics leading to long-lasting effects on specific brain gene regulation. To test this hypothesis, we used Zebrafish (Danio Rerio) as a model system. As previously shown, treatment with high doses of probiotics can modulate behavior in Zebrafish, causing significant changes in the expression of some brain-relevant genes, such as BDNF and Tph1A. Using an ultra-deep targeted analysis, we investigated the methylation state of the BDNF and Tph1A promoter region in the brain and gut of probiotic-treated and untreated Zebrafishes. Thanks to the high resolution power of our analysis, we evaluated cell-to-cell methylation differences. At this resolution level, we found slight DNA methylation changes in probiotic-treated samples, likely related to a subgroup of brain and gut cells, and that specific DNA methylation signatures significantly correlated with specific behavioral scores. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2218-273X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::661e661c0341c920cb4f5067b5990a84Test http://europepmc.org/articles/PMC7911505Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....661e661c0341c920cb4f5067b5990a84 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2218273X |
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