Development of an RT-LAMP Assay for the Rapid Detection of SFTS Virus
| العنوان: | Development of an RT-LAMP Assay for the Rapid Detection of SFTS Virus |
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| المؤلفون: | Takeshi Kurosu, Yuka Hamada, Tomoki Yoshikawa, Souichi Yamada, Satoko Sugimoto, Hitomi Kinoshita, Yuki Takamatsu, Masayuki Shimojima, Naoki Fujisawa, Shizuko Harada, Takayuki Sugimoto, Masayuki Saijo, Shoichi Toda, Shuetsu Fukushi, Shiori Sano |
| المصدر: | Viruses Volume 13 Issue 4 Viruses, Vol 13, Iss 693, p 693 (2021) |
| بيانات النشر: | MDPI, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Phlebovirus, 0301 basic medicine, Severe Fever with Thrombocytopenia Syndrome, genetic structures, 030106 microbiology, Loop-mediated isothermal amplification, Biology, Real-Time Polymerase Chain Reaction, Microbiology, Sensitivity and Specificity, Virus, Article, 03 medical and health sciences, Virology, Genotype, medicine, Humans, RT-LAMP, SFTS virus, simplified method, medicine.disease, biology.organism_classification, QR1-502, Reverse transcriptase, eye diseases, Severe fever with thrombocytopenia syndrome, 030104 developmental biology, Infectious Diseases, Molecular Diagnostic Techniques, Nucleic acid, RNA, Viral, sense organs, Primer (molecular biology), Nucleic Acid Amplification Techniques |
| الوصف: | Detection of severe fever with thrombocytopenia syndrome (SFTS) virus (SFTSV) during the early phase of the disease is important for appropriate treatment, infection control, and prevention of further transmission. The reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a nucleic acid amplification method that amplifies the target sequence under isothermal conditions. Here, we developed an RT-LAMP with a novel primer/probe set targeting a conserved region of the SFTSV L segment after extraction of viral RNA (standard RT-LAMP). Both the Chinese and Japanese SFTSV strains, including various genotypes, were detected by the standard RT-LAMP. We also performed RT-LAMP using the same primer/probe set but without the viral RNA extraction step (called simplified RT-LAMP) and evaluated the diagnostic efficacy. The sensitivity and specificity of the simplified RT-LAMP were 84.9% (45/53) and 89.5% (2/19), respectively. The simplified RT-LAMP can detect SFTSV in human sera containing > 103.5 copies/mL viral RNA. The two RT-LAMP positive but quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) negative samples were positive in the conventional RT-PCR, suggesting that there was no false positive reaction in the RT-LAMP. Both the standard and simplified RT-LAMP are useful for detecting the SFTSV genome in patients during the early phase of the disease. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1999-4915 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a9bcbecb7629b58842c88267e83c0b6Test http://europepmc.org/articles/PMC8073756Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6a9bcbecb7629b58842c88267e83c0b6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19994915 |
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