Organoids Are Limited in Modeling the Colon Adenoma–Carcinoma Sequence
| العنوان: | Organoids Are Limited in Modeling the Colon Adenoma–Carcinoma Sequence |
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| المؤلفون: | Manabu Futamura, Nobuhisa Matsuhashi, Yoshihisa Tokumaru, Kazuaki Takabe, Li Yan, Kazuhiro Yoshida, Wanqing Tian, Masanori Oshi, Ankit Patel |
| المصدر: | Cells Volume 10 Issue 3 Cells, Vol 10, Iss 488, p 488 (2021) |
| بيانات النشر: | MDPI, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Adenoma, Colorectal cancer, Colon Adenoma, organoid, T-Lymphocytes, colorectal cancer, Biology, Mechanistic Target of Rapamycin Complex 1, medicine.disease_cause, Models, Biological, Article, Cohort Studies, Lymphocytes, Tumor-Infiltrating, Cancer stem cell, medicine, Organoid, Tumor Microenvironment, Humans, lcsh:QH301-705.5, neoplasms, Cell Proliferation, Tumor microenvironment, B-Lymphocytes, GSEA, Macrophages, Immunity, Cancer, CIBERSORT, General Medicine, medicine.disease, digestive system diseases, Gene Expression Regulation, Neoplastic, Organoids, stomatognathic diseases, adenoma–carcinoma sequence, lcsh:Biology (General), Colonic Neoplasms, Cancer research, Neoplastic Stem Cells, xCell, Carcinogenesis |
| الوصف: | The colon adenoma–carcinoma sequence is a multistep genomic-altering process that occurs during colorectal cancer (CRC) carcinogenesis. Organoids are now commonly used to model both non-cancerous and cancerous tissue. This study aims to investigate how well organoids mimic tissues in the adenoma–carcinoma sequence by comparing their transcriptomes. A total of 234 tissue samples (48 adenomas and 186 CRC) and 60 organoid samples (15 adenomas and 45 CRC) were analyzed. We found that cell-proliferation-related gene sets were consistently enriched in both CRC tissues and organoids compared to adenoma tissues and organoids by gene set enrichment analysis (GSEA). None of the known pathways in the colon adenoma–carcinoma sequence were consistently enriched in CRC organoids. There was no enrichment of the tumor microenvironment-related gene sets in CRC organoids. CRC tissues enriched immune-response-related gene sets, whereas CRC organoids did not. The proportions of infiltrating immune cells were different between tissues and organoids, whereas there was no difference between cancer and adenoma organoids. The amounts of cancer stem cells and progenitor cells were not different between CRC and adenoma organoids, whereas a difference was noted between CRC and adenoma tissues. In conclusion, we demonstrated that organoids model only part of the adenoma–carcinoma sequence and should be used with caution after considering their limitations. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2073-4409 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a31b9a2223c08d7c1e9e9fce98571a0fTest http://europepmc.org/articles/PMC7996178Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a31b9a2223c08d7c1e9e9fce98571a0f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20734409 |
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