AT1 and AT2 receptor knockout changed osteonectin and bone density in mice in periodontal inflammation experimental model
| العنوان: | AT1 and AT2 receptor knockout changed osteonectin and bone density in mice in periodontal inflammation experimental model |
|---|---|
| المؤلفون: | Éricka Janine Dantas da Silveira, Alan Chan, Agnes Andrade Martins, Aurigena Antunes de Araújo, Luis J. Cruz, Gerly Anne de Castro Brito, Raimundo Fernandes de Araújo Júnior, Caroline Addison Carvalho Xavier de Medeiros, Renata Ferreira de Carvalho Leitão, Flavia Q. Pirih, Maria Laura de Souza Lima, Robson A.S. Santos, Vinícius Barreto Garcia, Gerlane Coelho Bernardo Guerra, Michael Bader |
| المصدر: | International Journal of Molecular Sciences, Vol 22, Iss 5217, p 5217 (2021) International Journal of Molecular Sciences International Journal of Molecular Sciences, 22(10). MDPI Volume 22 Issue 10 |
| بيانات النشر: | MDPI, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Lipopolysaccharides, Male, Bone density, Nitric Oxide Synthase Type II, bone, 0302 clinical medicine, Bone Density, Biology (General), micro-computed tomography, Receptor, periodontitis, Spectroscopy, Mice, Knockout, biology, osteonectin, Chemistry, Osteoblast, General Medicine, musculoskeletal system, Computer Science Applications, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Osteocalcin, cardiovascular system, medicine.symptom, Porphyromonas gingivalis, hormones, hormone substitutes, and hormone antagonists, circulatory and respiratory physiology, musculoskeletal diseases, medicine.medical_specialty, QH301-705.5, Inflammation, Receptor, Angiotensin, Type 2, Catalysis, Article, Receptor, Angiotensin, Type 1, Inorganic Chemistry, 03 medical and health sciences, Internal medicine, medicine, Animals, Femur, Physical and Theoretical Chemistry, Molecular Biology, QD1-999, Organic Chemistry, RANK Ligand, 030206 dentistry, X-Ray Microtomography, Disease Models, Animal, Endocrinology, Cardiovascular and Metabolic Diseases, inflammation, biology.protein, Osteonectin, Immunostaining |
| الوصف: | Background: The aim of this study was to evaluate the role of AT1 and AT2 receptors in a periodontal inflammation experimental model. Methods: Periodontal inflammation was induced by LPS/Porphyromonas gingivalis. Maxillae, femur, and vertebra were scanned using Micro-CT. Maxillae were analyzed histopathologically, immunohistochemically, and by RT-PCR. Results: The vertebra showed decreased BMD in AT1 H compared with WT H (p < 0.05). The femur showed increased Tb.Sp for AT1 H and AT2 H, p < 0.01 and p < 0.05, respectively. The Tb.N was decreased in the vertebra (WT H-AT1 H: p < 0.05 WT H-AT2 H: p < 0.05) and in the femur (WT H-AT1 H: p < 0.01 0.05). AT1 PD increased linear bone loss (p < 0.05) and decreased osteoblast cells (p < 0.05). RANKL immunostaining was intense for AT1 PD and WT PD (p < 0.001). OPG was intense in the WT H, WT PD, and AT2 PD when compared to AT1 PD (p < 0.001). AT1 PD showed weak immunostaining for osteocalcin compared with WT H, WT PD, and AT2 PD (p < 0.001). AT1 H showed significantly stronger immunostaining for osteonectin in fibroblasts compared to AT2 H (p < 0.01). Conclusion: AT1 receptor knockout changed bone density, the quality and number of bone trabeculae, decreased the number of osteoblast cells, and increased osteonectin in fibroblasts. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68cbf6270f5f9a17242b7214d4d7471eTest https://hdl.handle.net/1887/3277568Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....68cbf6270f5f9a17242b7214d4d7471e |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |