MicroRNA Profile of Patients with Chronic Limb-Threatening Ischemia
| العنوان: | MicroRNA Profile of Patients with Chronic Limb-Threatening Ischemia |
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| المؤلفون: | Shubha Jain, Mohammad Qadura, Rawand Abdin, Krishna K. Singh, Muzammil H. Syed, Abdelrahman Zamzam, Jason Valencia, Hamzah Khan |
| المصدر: | Diagnostics Diagnostics, Vol 10, Iss 230, p 230 (2020) Volume 10 Issue 4 |
| بيانات النشر: | MDPI, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Oncology, medicine.medical_specialty, Clinical Biochemistry, Ischemia, quantitative polymerase chain reaction, 030204 cardiovascular system & hematology, Article, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, microRNA, medicine, In patient, chronic limb threatening ischemia, next generation sequencing, lcsh:R5-920, business.industry, Confounding, Plasma levels, MicroRNA Profile, medicine.disease, body regions, 030104 developmental biology, Real-time polymerase chain reaction, Cohort, lcsh:Medicine (General), business |
| الوصف: | Chronic limb-threatening ischemia (CLTI) results in devastating complications such as lower-limb amputations. In this study, a genome-wide plasma microRNAs (miRNA) sequencing was performed to identify miRNA(s) associated with CLTI. Blood samples were collected from early stage CLTI patients (ABI < 0.9) and non-PAD controls (ABI &ge 0.9) for 3 experiments: discovery phase (n = 23), confirmatory phase (n = 52) and validation phase (n = 20). In the discovery phase, next generation sequencing (NGS) was used to identify miRNA circulating in the plasma CLTI (n = 13) patients, compared to non-PAD controls (n = 10). Two down-regulated miRNAs (miRNA-6843-3p and miRNA-6766-5p) and three upregulated miRNAs (miRNA-1827, miRNA-320 and miRNA-98-3p) were identified (&ge 2-fold change). In the confirmatory phase, these 5 deregulated miRNAs were further investigated in non-PAD (n = 21) and CTLI (n = 31) patients using qRT-PCR. Only miRNA-1827 was found to be significantly upregulated (&ge 3-fold, p-value < 0. 001) in the CLTI group. Lastly, to minimize the influence of confounding factors, miRNA-1827 plasma levels were validated in a third cohort of CLTI patients (n = 10) matched to non-PAD controls (n = 10). Our analysis demonstrated that miRNA-1827 expression was increased in the CLTI cohort (&ge 2-folds, p-value < 0.001). In summary, circulating miRNA-1827 is significantly elevated in patients with CLTI. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2075-4418 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0fbe8aa7b8d65202efd6d6598beb9784Test http://europepmc.org/articles/PMC7235988Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0fbe8aa7b8d65202efd6d6598beb9784 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20754418 |
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