Transcutaneous Administration of Dengue Vaccines
| العنوان: | Transcutaneous Administration of Dengue Vaccines |
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| المؤلفون: | Samuel Santos Pereira, Camila Mathias-Santos, Jaime Henrique Amorim, Lennon Ramos Pereira, Rúbens Prince dos Santos Alves, Carla Longo de Freitas, Aléxia Adrianne Venceslau-Carvalho, Sara Araujo Pereira, Robert Andreata-Santos, Maria Fernanda de Castro-Amarante, Marianna Teixeira de Pinho Favaro, Luís Carlos de Souza Ferreira |
| المصدر: | Viruses Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Viruses, Vol 12, Iss 514, p 514 (2020) Volume 12 Issue 5 |
| بيانات النشر: | MDPI, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, intradermic immunization, Injections, Intradermal, medicine.medical_treatment, lcsh:QR1-502, Dengue Vaccines, Dengue virus, medicine.disease_cause, Administration, Cutaneous, Antibodies, Viral, lcsh:Microbiology, Article, Dengue, 03 medical and health sciences, Mice, 0302 clinical medicine, Immune system, transcutaneous immunization, adjuvant, Virology, Enterotoxigenic Escherichia coli, medicine, Animals, Humans, heat-labile toxin, Dengue vaccine, Mice, Inbred BALB C, biology, business.industry, Immunogenicity, Dengue Virus, 030104 developmental biology, Infectious Diseases, Immunoglobulin G, Immunology, biology.protein, Cytokine secretion, Immunization, Antibody, business, Adjuvant, 030215 immunology, IMUNOGLOBULINAS |
| الوصف: | In the present study, we evaluated the immunological responses induced by dengue vaccines under experimental conditions after delivery via a transcutaneous (TC) route. Vaccines against type 2 Dengue virus particles (DENV2 New Guinea C (NGC) strain) combined with enterotoxigenic Escherichia coli (ETEC) heat-labile toxin (LT) were administered to BALB/c mice in a three-dose immunization regimen via the TC route. As a control for the parenteral administration route, other mouse groups were immunized with the same vaccine formulation via the intradermic (ID) route. Our results showed that mice vaccinated either via the TC or ID routes developed similar protective immunity, as measured after lethal challenges with the DENV2 NGC strain. Notably, the vaccine delivered through the TC route induced lower serum antibody (IgG) responses with regard to ID-immunized mice, particularly after the third dose. The protective immunity elicited in TC-immunized mice was attributed to different antigen-specific antibody properties, such as epitope specificity and IgG subclass responses, and cellular immune responses, as determined by cytokine secretion profiles. Altogether, the results of the present study demonstrate the immunogenicity and protective properties of a dengue vaccine delivered through the TC route and offer perspectives for future clinical applications. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1999-4915 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c9a2656597d18f9cafa55bfb15299fbTest http://europepmc.org/articles/PMC7290698Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5c9a2656597d18f9cafa55bfb15299fb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19994915 |
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