دورية أكاديمية
Type IV collagen and SOX9 are molecular targets of BET inhibition in experimental glomerulosclerosis
| العنوان: | Type IV collagen and SOX9 are molecular targets of BET inhibition in experimental glomerulosclerosis |
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| المؤلفون: | Morgado-Pascual, José Luis, Suárez-Álvarez, Beatriz, Marchant, Vanessa, Basantes, Pamela, Tharaux, Pierre-Louis, Ortiz Arduán, Alberto, López-Larrea, Carlos, Ruiz Ortega, Marta, Rayego-Mateos, Sandra |
| المساهمون: | UAM. Departamento de Medicina |
| بيانات النشر: | MDPI |
| سنة النشر: | 2023 |
| المجموعة: | Universidad Autónoma de Madrid (UAM): Biblos-e Archivo |
| مصطلحات موضوعية: | glomerular diseases, BET, fibrosis, SOX9, epigenetic, kidney, Medicina |
| الوصف: | Progressive glomerulonephritis (GN) is characterized by an excessive accumulation of extracellular (ECM) proteins, mainly type IV collagen (COLIV), in the glomerulus leading to glomerulosclerosis. The current therapeutic approach to GN is suboptimal. Epigenetic drugs could be novel therapeutic options for human disease. Among these drugs, bromodomain and extra-terminal domain (BET) inhibitors (iBETs) have shown beneficial effects in experimental kidney disease and fibrotic disorders. Sex-determining region Y-box 9 (SOX9) is a transcription factor involved in regulating proliferation, migration, and regeneration, but its role in kidney fibrosis is still unclear. We investigated whether iBETs could regulate ECM accumulation in experimental GN and evaluated the role of SOX9 in this process. For this purpose, we tested the iBET JQ1 in mice with anti-glomerular basement membrane nephritis induced by nephrotoxic serum (NTS). In NTS-injected mice, JQ1 treatment reduced glomerular ECM deposition, mainly by inhibiting glomerular COLIV accumulation and Col4a3 gene overexpression. Moreover, chromatin immunoprecipitation assays demonstrated that JQ1 inhibited the recruitment and binding of BRD4 to the Col4a3 promoter and reduced its transcription. Active SOX9 was found in the nuclei of glomerular cells of NTS-injured kidneys, mainly in COLIV-stained regions. JQ1 treatment blocked SOX9 nuclear translocation in injured kidneys. Moreover, in vitro JQ1 blocked TGF-β1-induced SOX9 activation and ECM production in cultured mesangial cells. Additionally, SOX9 gene silencing inhibited ECM production, including COLIV production. Our results demonstrated that JQ1 inhibited SOX9/COLIV, to reduce experimental glomerulosclerosis, supporting further research of iBET as a potential therapeutic option in progressive glomerulosclerosis ; This research was funded by grants from the Instituto de Salud Carlos III (ISCIII) and Fondos FEDER European Union (PI20/00140, PI19/00815, and DTS20/00083). Red de Investigación Renal REDINREN: ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | International Journal of Molecular Sciences; https://doi.org/10.3390/ijms24010486Test; Gobierno de España. PI20/00140; Gobierno de España. PI19/00815; Gobierno de España. DTS20/00083; International Journal of Molecular Sciences 24.1 (2023): 486; 1422-0067 (online); 1661-6596 (print); http://hdl.handle.net/10486/708953Test; 486-1; 486-20; 24 |
| DOI: | 10.3390/ijms24010486 |
| الإتاحة: | https://doi.org/10.3390/ijms24010486Test http://hdl.handle.net/10486/708953Test |
| حقوق: | © 2022 by the authors ; Reconocimiento ; openAccess |
| رقم الانضمام: | edsbas.326759D6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/ijms24010486 |
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