Stem Cells from a Female Rat Model of Type 2 Diabetes/Obesity and Stress Urinary Incontinence Are Damaged by In Vitro Exposure to its Dyslipidemic Serum, Predicting Inadequate Repair Capacity In Vivo
| العنوان: | Stem Cells from a Female Rat Model of Type 2 Diabetes/Obesity and Stress Urinary Incontinence Are Damaged by In Vitro Exposure to its Dyslipidemic Serum, Predicting Inadequate Repair Capacity In Vivo |
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| المؤلفون: | Istvan Kovanecz, Robert Gelfand, Alec Ohanian, Randy Ricks, Tom F. Lue, Nestor F. Gonzalez-Cadavid, Sheila Sharifzad, Guiting Lin |
| المصدر: | International Journal of Molecular Sciences International journal of molecular sciences, vol 20, iss 16 Volume 20 Issue 16 International Journal of Molecular Sciences, Vol 20, Iss 16, p 4044 (2019) |
| بيانات النشر: | MDPI, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Aging, medicine.medical_treatment, 030232 urology & nephrology, Apoptosis, Myostatin, Type 2 diabetes, Regenerative Medicine, wound closure, lcsh:Chemistry, 0302 clinical medicine, 2.1 Biological and endogenous factors, Aetiology, lcsh:QH301-705.5, Spectroscopy, Cells, Cultured, Cultured, biology, microRNA, Stem Cells, Diabetes, apoptosis, General Medicine, Stem-cell therapy, Computer Science Applications, Zucker, myostatin, fat infiltration, Female, Stem cell, Development of treatments and therapeutic interventions, Type 2, Biotechnology, Urologic Diseases, medicine.medical_specialty, muscle-derived stem cells, Cells, Catalysis, Article, Inorganic Chemistry, 03 medical and health sciences, In vivo, Internal medicine, medicine, Diabetes Mellitus, Genetics, Animals, Obesity, Physical and Theoretical Chemistry, Interleukin 6, Molecular Biology, Metabolic and endocrine, Nutrition, Dyslipidemias, Chemical Physics, 5.2 Cellular and gene therapies, business.industry, Animal, interleukin-6, Organic Chemistry, dyslipidemia, medicine.disease, Stem Cell Research, In vitro, Rats, Rats, Zucker, Disease Models, Animal, 030104 developmental biology, Endocrinology, Urinary Incontinence, lcsh:Biology (General), lcsh:QD1-999, Diabetes Mellitus, Type 2, Disease Models, biology.protein, Other Biological Sciences, business, Other Chemical Sciences, Stem Cell Transplantation |
| الوصف: | Female stress urinary incontinence (FSUI) is prevalent in women with type 2 diabetes/obesity (T2D/O), and treatment is not optimal. Autograph stem cell therapy surprisingly has poor efficacy. In the male rat model of T2D/O, it was demonstrated that epigenetic changes, triggered by long-term exposure to the dyslipidemic milieu, led to abnormal global transcriptional signatures (GTS) of genes and microRNAs (miR), and impaired the repair capacity of muscle-derived stem cells (MDSC). This was mimicked in vitro by treatment of MDSC with dyslipidemic serum or lipid factors. The current study aimed to predict whether these changes also occur in stem cells from female 12 weeks old T2D/O rats, a model of FSUI. MDSCs from T2D/O (ZF4-SC) and normal female rats (ZL4-SC) were treated in vitro with either dyslipidemic serum (ZFS) from late T2D/O 24 weeks old female Zucker fatty (ZF) rats, or normal serum (ZLS) from 24 weeks old female Zucker lean (ZL) rats, for 4 days and subjected to assays for fat deposition, apoptosis, scratch closing, myostatin, interleukin-6, and miR-GTS. The dyslipidemic ZFS affected both female stem cells more severely than in the male MDSC, with some gender-specific differences in miR-GTS. The changes in miR-GTS and myostatin/interleukin-6 balance may predict in vivo noxious effects of the T2D/O milieu that might impair autograft stem cell (SC) therapy for FSUI, but this requires future studies. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::622699b20018b7c9cd0928c1bbefa496Test http://europepmc.org/articles/PMC6720976Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....622699b20018b7c9cd0928c1bbefa496 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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