المؤلفون: Pambet, Mathilde¹ (AUTHOR), Sirodot, Fanny¹ (AUTHOR), Pereira, Bruno² (AUTHOR), Cahierc, Romain¹ (AUTHOR), Delabaere, Amélie^1,3 (AUTHOR), Comptour, Aurélie¹ (AUTHOR), Rouzaire, Marion¹ (AUTHOR), Sapin, Vincent^4,5 (AUTHOR), Gallot, Denis^1,5 (AUTHOR) dgallot@chu-clermontferrand.fr

المصدر: Journal of Clinical Medicine. Sep2023, Vol. 12 Issue 17, p5707. 11p.

مصطلحات موضوعية: *PREMATURE labor, *CHILDBIRTH, *UNIVERSITY hospitals, *FIBRONECTINS, *LONGITUDINAL method

مصطلحات جغرافية: CLERMONT-Ferrand (France)

مستخلص: We conducted a prospective double-blind study to compare two vaginal diagnostic methods in singleton pregnancies with threatened preterm labor (TPL) at the University Hospital of Clermont-Ferrand (France) from August 2018 to December 2020. Our main objective was to compare the diagnostic capacity at admission, in terms of positive predictive value (PPV) and negative predictive value (NPV), of Premaquick® (combined detection of IL-6/total IGFBP-1/native IGFBP-1) and QuikCheck fFN™ (fetal fibronectin) for delivery within 7 days in cases of TPL. We included 193 patients. Premaquick® had a sensitivity close to 89%, equivalent to QuikCheck fFN™, but a higher statistical specificity of 49.5% against 38.6% for QuikCheck fFN™. We found no superiority of Premaquick® over QuickCheck fFN™ in terms of PPV (6.6% vs. 7.9%), with NPV being equivalent in predicting childbirth within 7 days in cases of TPL (98.6% vs. 98.9%). Nevertheless, the combination of positive native and total IGFBP-1 and the combination of all three positive markers were associated with a higher PPV. Our results, though non-significant, support this combined multiple-biomarker approach to improve testing in terms of predictive values. [ABSTRACT FROM AUTHOR]

المؤلفون: Flis, Wojciech, Socha, Maciej W.

المصدر: Cells (2073-4409); Apr2024, Vol. 13 Issue 7, p600, 22p

مصطلحات موضوعية: FRUIT ripening, NLRP3 protein, CHILDBIRTH, INFLAMMASOMES, PREMATURE labor, CERVIX uteri, INFLAMMATORY mediators

مستخلص: The uterine cervix is one of the key factors involved in ensuring a proper track of gestation and labor. At the end of the gestational period, the cervix undergoes extensive changes, which can be summarized as a transformation from a non-favorable cervix to one that is soft and prone to dilation. During a process called cervical ripening, fundamental remodeling of the cervical extracellular matrix (ECM) occurs. The cervical ripening process is a derivative of many interlocking and mutually driving biochemical and molecular pathways under the strict control of mediators such as inflammatory cytokines, nitric oxide, prostaglandins, and reactive oxygen species. A thorough understanding of all these pathways and learning about possible triggering factors will allow us to develop new, better treatment algorithms and therapeutic goals that could protect women from both dysfunctional childbirth and premature birth. This review aims to present the possible role of the NLRP3 inflammasome in the cervical ripening process, emphasizing possible mechanisms of action and regulatory factors. [ABSTRACT FROM AUTHOR]

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المؤلفون: Adamczak, Ada Maria, Werblińska, Alicja, Jamka, Małgorzata, Walkowiak, Jarosław

المصدر: Biomedicines; Mar2024, Vol. 12 Issue 3, p490, 19p

مصطلحات موضوعية: GUT microbiome, PREMATURE labor, HUMAN microbiota, CHILDREN'S health

مستخلص: In recent years, the number of scientific publications on the role of intestinal microbiota in shaping human health, as well as the occurrence of intestinal dysbiosis in various disease entities, has increased dynamically. However, there is a gap in comprehensively understanding the factors influencing a child's gut microbiota. This review discusses the establishment of gut microbiota and the immunological mechanisms regulating children's microbiota, emphasising the importance of prioritising the development of appropriate gut microbiota in a child from the planning stages of pregnancy. The databases PubMed, Web of Sciences, Cochrane, Scopus and Google Scholar were searched to identify relevant articles. A child's gut microbiota composition is influenced by numerous factors, such as diet during pregnancy, antibiotic therapy, the mother's vaginal microbiota, delivery method, and, later, feeding method and environmental factors. During pregnancy, the foetus naturally acquires bacterial strains from the mother through the placenta, thereby shaping the newborn's immune system. Inappropriate maternal vaginal microbiota may increase the risk of preterm birth. Formula-fed infants typically exhibit a more diverse microbiota than their breastfed counterparts. These factors, among others, shape the maturation of the child's immune system, impacting the production of IgA antibodies that are central to cellular humoral immune defence. Further research should focus on identifying specific microbiota–immune system interactions influencing a child's immune health and developing personalised treatment strategies for immune-related disorders. [ABSTRACT FROM AUTHOR]

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المؤلفون: Khayargoli, Pranamika, Mayrand, Marie-Hélène, Niyibizi, Joseph, Audibert, François, Laporte, Louise, Lacaille, Julie, Carceller, Ana Maria, Lacroix, Jacques, Comète, Émilie, Coutlée, François, Trottier, Helen

المصدر: Viruses (1999-4915); Feb2024, Vol. 16 Issue 2, p298, 13p

مصطلحات موضوعية: HUMAN papillomavirus, VIRAL load, PREGNANCY, PREMATURE labor, FIRST trimester of pregnancy, THIRD trimester of pregnancy, CHILDBIRTH

مستخلص: Recent evidence shows increased preterm birth risk with human papillomavirus-16 (HPV16) infection during pregnancy. This study aimed to measure the association between HPV16 viral load during pregnancy and preterm birth. We used data from participants in the HERITAGE study. The Linear Array assay was used for HPV DNA testing on vaginal samples collected during the first and third trimesters of pregnancy. The HPV16 viral load was measured with a real-time polymerase chain reaction. We used logistic regression to measure the associations between HPV16 viral load during pregnancy and preterm birth (defined as birth before 37 weeks of gestation). The adjusted odd ratios (aORs) and the 95% confidence intervals [CIs] were estimated with inverse probability treatment weighting of the propensity score. This study included 48 participants who tested positive for HPV16 during the first trimester of pregnancy. The aOR for the association between first-trimester HPV16 viral load (higher viral load categorized with a cutoff of 0.5 copy/cell) was 13.04 [95% CI: 1.58–107.57]). Similar associations were found using different cutoffs for the categorization of viral load during the first and third trimesters. Our findings suggest a strong association between a high HPV16 viral load during pregnancy and preterm birth, demonstrating a biological gradient that reinforces the biological plausibility of a causal association. [ABSTRACT FROM AUTHOR]

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المؤلفون: Gruber, Teresa Mira, Ortlieb, Laura, Henrich, Wolfgang, Mechsner, Sylvia

المصدر: Journal of Clinical Medicine; Jan2024, Vol. 13 Issue 2, p414, 11p

مصطلحات موضوعية: PREGNANCY complications, CHILDBIRTH, PLACENTA praevia, PREMATURE labor, MATERNAL age, ENDOMETRIOSIS, PELVIC pain

مستخلص: Women with endometriosis (EM), particularly the manifestations of adenomyosis (AM) and deep infiltrating endometriosis (DIE), suffer from pain and sterility. DIE also appears with several specific obstetric complications. To determine the risk profile, we designed a retrospective case–control study. Primary outcomes were defined as the risk of preterm birth and caesarean delivery (CD). Primiparous singleton pregnancies in women with DIE were compared with controls without EM. We matched for mode of conception and maternal age. A total of 41 women diagnosed with DIE and 164 controls were recruited. A total of 92.7% of the cases were also diagnosed with AM. Preterm birth occurred in 12.2% of cases and in 6.7% of controls. The difference was not statistically significant (OR: 1.932; 95% CI: 0.632–5.907). The rate of CD was similar in both groups. Remarkably, placental implantation disorders in the form of placenta praevia were eight times more frequent in women with DIE (9.8%) than in controls (1.2%, OR: 8.757; 95% CI: 1.545–49.614). Neonatal outcome was similar in both groups. Four out of fourteen cases reported abdominal wall endometriosis after CD. Women with DIE/AM and with placenta praevia are at risk of bleeding complications. After CD, they can develop abdominal wall EM. We therefore suggest evaluating the birth mode in each woman with DIE/AM. [ABSTRACT FROM AUTHOR]

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المؤلفون: Gil-Kulik, Paulina, Leśniewski, Michał, Bieńko, Karolina, Wójcik, Monika, Więckowska, Marta, Przywara, Dominika, Petniak, Alicja, Kondracka, Adrianna, Świstowska, Małgorzata, Szymanowski, Rafał, Wilińska, Agnieszka, Wiliński, Mateusz, Płachno, Bartosz J., Kostuch, Marzena, Rahnama-Hezavach, Mansur, Szuta, Mariusz, Kwaśniewska, Anna, Bogucka-Kocka, Anna, Kocki, Janusz

المصدر: International Journal of Molecular Sciences; Feb2023, Vol. 24 Issue 3, p2476, 20p

مصطلحات موضوعية: GENE expression, PERINATAL death, BREAST milk, STEM cells, MATERNAL age, MESENCHYMAL stem cells, PREMATURE labor, CHILDBIRTH, IMMUNOLOGIC diseases

مستخلص: Due to their therapeutic potential, mesenchymal stem cells are the subject of intensive research on the use of their potential in the treatment of, among others, neurodegenerative diseases or immunological diseases. They are among the newest in the field of medicine. The presented study aimed to evaluate the expression of eight genes from the IAP family and the gene regulating IAP—XAF1—in stem cells derived from human milk, using the qPCR method. The relationships between the expression of genes under study and clinical data, such as maternal age, maternal BMI, week of pregnancy in which the delivery took place, bodyweight of the newborn, the number of pregnancies and deliveries, and the time elapsed since delivery, were also analyzed. The research was carried out on samples of human milk collected from 42 patients hospitalized in The Clinic of Obstetrics and Perinatology of the Independent Public Clinical Hospital No. 4, in Lublin. The conducted research confirmed the expression of the following genes in the tested material: NAIP, BIRC2, BIRC3, BIRC5, BIRC6, BIRC8, XIAP, XAF1, OCT4 and SOX2. Moreover, several dependencies of the expression of individual genes on the maternal BMI (BIRC5, XAF1 and NAIP), the time since childbirth (BIRC5, BIRC6, XAF1 and NAIP), the number of pregnancies and deliveries (BIRC2, BIRC5, BIRC6 and XAF1), the manner of delivery (XAF1 and OCT4), preterm labor (BIRC6 and NAIP) were demonstrated. Additionally, we found positive relationships between gene expression of BIRC7, BIRC8 and XAF1 and the main factors of pluripotency: SOX2 and OCT4. This work is the first to investigate the expression of genes from the IAPs family in mother's milk stem cells. [ABSTRACT FROM AUTHOR]

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المؤلفون: Teraoka, Yuko, Sugimoto, Jun, Konishi, Haruhisa, Miyoshi, Hiroshi, Furusho, Hisako, Miyauchi, Mutsumi, Kajioka, Shunichi, Koh, Iemasa, Kudo, Yoshiki

المصدر: Biomolecules (2218-273X); Aug2022, Vol. 12 Issue 8, p1029, 10p

مصطلحات موضوعية: UTERINE contraction, PORPHYROMONAS gingivalis, PREMATURE labor, CHILDBIRTH, PROGESTERONE, CONTRACTILITY (Biology)

مستخلص: Preterm birth is one of the most significant obstetric complications. Inflammation reportedly promotes uterine contraction and weakening of the fetal membrane, which induces preterm birth. Previous studies using animal models of lipopolysaccharide-induced acute inflammation have shown that progesterone (P4) promotes uterine quiescence. However, this effect is not fully understood in chronic inflammation. This study aimed to investigate the effects of P4 on uterine contractility and inflammation of the fetal membrane in mice infected with Porphyromonas gingivalis (P.g.), a major periodontal pathogen as a model of preterm birth caused by chronic inflammation. Mice were injected with 1 mg of P4 from day 15.5 to 17.5. P4 prolonged the mean gestation period of P.g mice from 18.3 to 20.4 days, and no reduction in the gestation period was observed. P4 treatment suppressed spontaneous uterine contractility and decreased oxytocin sensitivity. In addition, the expression of inflammatory cytokines in the fetal membrane was significantly reduced. Thus, P4 prevented preterm birth by suppressing enhanced uterine contractility induced by chronic inflammation in this model. This result describes the effects of P4 in a chronic inflammation model, which may lead to a better understanding of the efficacy of P4 in preventing preterm birth in humans. [ABSTRACT FROM AUTHOR]

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المؤلفون: Gródecka-Szwajkiewicz, Dorota¹ (AUTHOR) kawamilosz@gmail.com, Ulańczyk, Zofia¹ (AUTHOR), Zagrodnik, Edyta¹ (AUTHOR), Łuczkowska, Karolina¹ (AUTHOR), Rogińska, Dorota¹ (AUTHOR), Kawa, Miłosz P.¹ (AUTHOR), Stecewicz, Iwona¹ (AUTHOR), Safranow, Krzysztof² (AUTHOR), Machaliński, Bogusław¹ (AUTHOR) machalin@pum.edu.pl

المصدر: International Journal of Molecular Sciences. Feb2020, Vol. 21 Issue 4, p1305. 1p.

مصطلحات موضوعية: *CORD blood, *PREMATURE labor, *SECRETION, *CHILDBIRTH, *MICRORNA, *PREMATURE infants

مستخلص: Objectives: Premature birth, defined as less than 37 weeks gestation, affects approximately 12% of all live births around the world. Advances in neonatal care have resulted in the increased survival of infants born prematurely. Although prematurity is a known risk factor for different cardiovascular diseases, little is known about the pathophysiology of vasculature during premature gestation and angiopoietic factors network during premature birth. Aims: The objective of this study was to determine whether the profile of several pro-angiogenic and anti-angiogenic factors in umbilical cord blood (UCB) is different in healthy appropriate-for-gestational-age preterm newborns and normal term babies. The second aim of this study was to investigate the microRNA (miRNAs) expression profile in UCB from preterm labor and to detect miRNAs potentially taking part in control of angogenesis-related processes (Angio-MiRs). Methods: Using an immunobead Luminex assay, we simultaneously measured the concentration of Angiogenin, Angiopoietin-1, FGF-acidic, FGF-basic, PDGF-aa, PlGF, VEGF, VEGF-D, Endostatin, Thrombospondin-2, NGF, BDNF, GDNF, and NT-4 in UCB samples collected from the preterm (n = 27) and term (n = 52) delivery. In addition, the global microRNA expression in peripheral blood mononuclear cells (PBMCs) circulating in such UCB samples was examined in this study using microarray MiRNA technique. Results: The concentrations of five from eight measured pro-angiogenic factors (VEGF, Angiopoietin-1, PDGF-AA, FGF-a, and FGF-b) were significantly lower in UCB from preterm newborns. On the contrary, two angiostatic factors (Endostatin and Thrombospondin-2) were significantly up-regulated in preterm UCB. Among analyzed neurotrophins in preterm newborns, the elevated UCB concentration was found only in the case of GDNF, whereas BDNF was significantly reduced. Moreover, two angiopoietic factors, VEGF-D and PlGF, and two neurotrophins, NT4 and NGF, did not differ in concentration in preterm and term babies. We also discovered that among the significantly down-regulated miRNAs, there were several classical Angio-MiRs (inter alia MiR-125, MiR-126, MiR-145, MiR-150, or MiR155), which are involved in angiogenesis regulation in newborn after preterm delivery. Conclusions: This is the first report of simultaneous measurements of several angiopoietic factors in UCB collected from infants during preterm and term labor. Here, we observed that several pro-angiogenic factors were at lower concentration in UCB collected from preterm newborns than term babies. In contrast, the two measured angiostatic factors, Endostatin and Thrombospondin-2, were significantly higher in UCB from preterm babies. This can suggest that distinct pathophysiological contributions from differentially expressed various angiopoietic factors may determine the clinical outcomes after preterm birth. Especially, our angiogenesis-related molecules analysis indicates that preterm birth of healthy, appropriate-for-gestational-age newborns is an "anti-angiogenic state" that may provide an increased risk for improper development and function of cardiovascular system in the adulthood. This work also contributes to a better understanding of the role of miRNAs potentially involved in angiogenesis control in preterm newborns. [ABSTRACT FROM AUTHOR]