YB-1 Is Altered in Pregnancy-Associated Disorders and Affects Trophoblast in Vitro Properties via Alternation of Multiple Molecular Traits
| العنوان: | YB-1 Is Altered in Pregnancy-Associated Disorders and Affects Trophoblast in Vitro Properties via Alternation of Multiple Molecular Traits |
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| المؤلفون: | Florence Fischer, Peter R. Mertens, Katja Woidacki, Violeta Stojanovska, Mario Bauer, Ana Claudia Zenclussen, Jonathan A. Lindquist, Aneri Shah |
| المصدر: | International Journal of Molecular Sciences Volume 22 Issue 13 International Journal of Molecular Sciences, Vol 22, Iss 7226, p 7226 (2021) |
| بيانات النشر: | MDPI, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, NF-κB, chemistry.chemical_compound, 0302 clinical medicine, Pre-Eclampsia, Cell Movement, Pregnancy, Biology (General), Spectroscopy, reproductive and urinary physiology, Gene knockdown, Fetal Growth Retardation, cold shock protein, NF-kappa B, apoptosis, General Medicine, Computer Science Applications, Cell biology, Trophoblasts, Up-Regulation, Chemistry, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Gene Knockdown Techniques, embryonic structures, Female, medicine.symptom, Adult, intrauterine growth restriction, QH301-705.5, placentation, Down-Regulation, Inflammation, Biology, In Vitro Techniques, Catalysis, Article, Cell Line, Inorganic Chemistry, preeclampsia, 03 medical and health sciences, Young Adult, Downregulation and upregulation, medicine, Humans, Secretion, RNA, Messenger, Physical and Theoretical Chemistry, Molecular Biology, QD1-999, Cell Proliferation, Organic Chemistry, Trophoblast, Placentation, Abortion, Spontaneous, Pregnancy Complications, 030104 developmental biology, chemistry, Case-Control Studies, Cancer cell, Y-Box-Binding Protein 1 |
| الوصف: | Cold shock Y-box binding protein-1 (YB-1) coordinates several molecular processes between the nucleus and the cytoplasm and plays a crucial role in cell function. Moreover, it is involved in cancer progression, invasion, and metastasis. As trophoblast cells share similar characteristics with cancer cells, we hypothesized that YB-1 might also be necessary for trophoblast functionality. In samples of patients with intrauterine growth restriction, YB-1 mRNA levels were decreased, while they were increased in preeclampsia and unchanged in spontaneous abortions when compared to normal pregnant controls. Studies with overexpression and downregulation of YB-1 were performed to assess the key trophoblast processes in two trophoblast cell lines HTR8/SVneo and JEG3. Overexpression of YB-1 or exposure of trophoblast cells to recombinant YB-1 caused enhanced proliferation, while knockdown of YB-1 lead to proliferative disadvantage in JEG3 or HTR8/SVneo cells. The invasion and migration properties were affected at different degrees among the trophoblast cell lines. Trophoblast expression of genes mediating migration, invasion, apoptosis, and inflammation was altered upon YB-1 downregulation. Moreover, IL-6 secretion was excessively increased in HTR8/SVneo. Ultimately, YB-1 directly binds to NF-κB enhancer mark in HTR8/SVneo cells. Our data show that YB-1 protein is important for trophoblast cell functioning and, when downregulated, leads to trophoblast disadvantage that at least in part is mediated by NF-κB. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06de04b8ef3a0fd01de6d9d7d195c235Test http://europepmc.org/articles/PMC8269420Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....06de04b8ef3a0fd01de6d9d7d195c235 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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