دورية أكاديمية
Evaluation of the effect of crocetin on antitumor activity of doxorubicin encapsulated in PLGA nanoparticles
| العنوان: | Evaluation of the effect of crocetin on antitumor activity of doxorubicin encapsulated in PLGA nanoparticles |
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| المؤلفون: | F. A Langroodi, Z. Hafezi Ghahestani, M. Alibolandi, M. Ebrahimian, M. Hashemi |
| المصدر: | Nanomedicine Journal, Vol 3, Iss 1, Pp 23-34 (2016) |
| بيانات النشر: | Mashhad University of Medical Sciences, 2016. |
| سنة النشر: | 2016 |
| المجموعة: | LCC:Medicine (General) |
| مصطلحات موضوعية: | Breast cancer, Chemotherapy, Crocetin, Doxorubicin, PLGA, Medicine (General), R5-920 |
| الوصف: | Objective(s): The current study reports investigation of codelivery by PLGA nanoparticles (NPs) loaded with crocetin (Cro), a natural carotenoid dicarboxylicHYPERLINK “http://en.wikipedia.org/wiki/Carboxylic_acidTest” acid that is found in the crocus flower, and Doxorubicin (DOX). Materials and Methods: Double emulsion/solvent evaporation method was used for preparation of PLGA nanoparticles containing Dox and Cro. Characterizations of prepared NPs were investigated by atomic force microscopy (AFM) and dynamic light scattering analysis. In vitro Cytotoxicity of DOX and Cro loaded PLGA NPs (PLGA-DOX-Cro) on MCF-7 cell line was evaluated using MTT test. Flow cytometry experiments were implemented to distinguish cells undergoing apoptosis from those undergoing necrosis. Furthermore the expression of caspase 3 was examined by western blot analysis. Results: The prepared formulations had size of 150- 300 nm. Furthermore, PLGA-DOX-Cro nanoparticles inhibited MCF-7 tumor cells growth more efficiently than either DOX or Cro alone at the same concentrations, as quantified by MTT assay and flow cytometry. Studies on cellular uptake of DOX-Cro-NPs demonstrated that NPs were effectively taken up by MCF-7 tumor cells. Conclusion: This study suggested that DOX-Cro-NPs may have promising applications in breast cancer therapy. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2322-3049 2322-5904 |
| العلاقة: | http://nmj.mums.ac.ir/article_6193_fa5ebe8191f7d7db5d56b8e8f89cb275.pdfTest; https://doaj.org/toc/2322-3049Test; https://doaj.org/toc/2322-5904Test |
| DOI: | 10.7508/nmj.2016.01.003 |
| الوصول الحر: | https://doaj.org/article/85d7128bae9f46e7b90574a5475f176bTest |
| رقم الانضمام: | edsdoj.85d7128bae9f46e7b90574a5475f176b |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 23223049 23225904 |
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| DOI: | 10.7508/nmj.2016.01.003 |