Assessing Urine Human Papillomavirus Polymerase Chain Reaction Testing As a Tool for Screening Anal HPV Infection in HIV-Positive MSM
| العنوان: | Assessing Urine Human Papillomavirus Polymerase Chain Reaction Testing As a Tool for Screening Anal HPV Infection in HIV-Positive MSM |
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| المؤلفون: | Jean-Luc Schmit, Bernadette Fouche, Youcef Douadi, Christine Robin, Jean-Philippe Lanoix, Marine Woimant, Henri Sevestre, Gilles Duverlie, Alice Borel, Youssef El Samad, Olivier Ganry, Christine Pannier, Caroline Lecaque |
| المصدر: | AIDS Patient Care and STDs. 26:208-213 |
| بيانات النشر: | Mary Ann Liebert Inc, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Adult, Male, medicine.medical_specialty, Sexual Behavior, Anal Canal, Urine, Real-Time Polymerase Chain Reaction, Gastroenterology, Men who have sex with men, Predictive Value of Tests, Surveys and Questionnaires, Internal medicine, HIV Seropositivity, Prevalence, medicine, Humans, Homosexuality, Male, Papillomaviridae, Gynecology, Anus Diseases, business.industry, Papillomavirus Infections, Public Health, Environmental and Occupational Health, HPV infection, virus diseases, Middle Aged, Viral Load, Anal Infection, medicine.disease, CD4 Lymphocyte Count, Infectious Diseases, Real-time polymerase chain reaction, Predictive value of tests, Population study, France, business, Viral load |
| الوصف: | Multiple types of human papillomavirus (HPV) are responsible for most cervical cancers but also cause anal cancers-especially in HIV-positive patients. Furthermore, men who have sex with men (MSM) are twice as likely to develop anal cancers as non-MSM. A simple screening test for HPV infection would be useful in these patients. The aim of our study was to evaluate the detection of HPV by real-time polymerase chain reaction (PCR) in urine as a marker of anal infection in MSM. The study included 52 HIV-positive MSM treated at Amiens University Hospital (Amiens, France). After obtaining informed consent, we performed an anal swab and gathered 10 mL of first-void urine. Samples were extracted and amplified in a real-time PCR. Genotypes were determined with a PapilloCheck(®) system (Greiner Bio-One, Frickenhausen, Germany). The anal test was the gold standard for calculating the characteristics of the urine test. The sensitivity of the urine test for diagnosing anal HPV infection was 15%, the specificity was 66%, the positive predictive value was 87.5%, and negative predictive value was 4.5%. The prevalence of anal HPV infection in the study population was 94%. Genotype 42 was the most common. The anal HPV viral load was significantly lower in men in a stable relationship than in single men. However, there was no statistically significant relationship between anal viral load and anal intraepithelial lesions. We conclude that urine-based HPV is a poor predictor of anal HPV infection in HIV-positive MSM. |
| تدمد: | 1557-7449 1087-2914 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53a098d464dc3a8b59e5c651197c6150Test https://doi.org/10.1089/apc.2011.0301Test |
| رقم الانضمام: | edsair.doi.dedup.....53a098d464dc3a8b59e5c651197c6150 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15577449 10872914 |
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