Endoderm and Pancreatic Islet Lineage Differentiation from Human Embryonic Stem Cells
| العنوان: | Endoderm and Pancreatic Islet Lineage Differentiation from Human Embryonic Stem Cells |
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| المؤلفون: | Brenda Kahan, Lynn M. Jacobson, Victoria L. Browning, Jon S. Odorico, Andrea Forgianni, Nathan R. Treff, Peicheng Jing, Xiaofang Xu |
| المصدر: | Cloning and Stem Cells. 8:96-107 |
| بيانات النشر: | Mary Ann Liebert Inc, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | medicine.medical_specialty, Time Factors, Cellular differentiation, Cell Culture Techniques, Embryoid body, Biology, Cell fate commitment, Islets of Langerhans, Internal medicine, medicine, Humans, Cell Lineage, Progenitor cell, Cells, Cultured, reproductive and urinary physiology, Stem Cells, Endoderm, Gene Expression Regulation, Developmental, Cell Differentiation, Embryonic stem cell, Cell biology, Transplantation, medicine.anatomical_structure, Endocrinology, embryonic structures, biological phenomena, cell phenomena, and immunity, Biomarkers, Developmental Biology, Biotechnology, Definitive endoderm |
| الوصف: | Human embryonic stem cells (HESCs) are a potential source of insulin-producing tissue for transplantation. Recent studies have begun to define factors that promote definitive endoderm formation from HESCs, but conditions permitting complete islet specification in vitro have not been described. Here, we study spontaneous differentiation of HESCs to definitive endoderm and pancreatic progenitor cells, and begin to determine which aspects of the protocol are required for this cell fate commitment. HESCs were differentiated in culture for up to 10 weeks, including an embryoid body (EB) formation step. Modifications to the protocol included elimination of the EB phase, varying initial cell cluster size when forming EBs, and addition of mesoderm-derived cells to EBs. Differentiated cells were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. HESCs are capable of spontaneous differentiation to cells expressing the definitive endoderm and pancreatic progenitor markers Foxa2, Sox17, and Pdx1, and ultimately, some cells express islet endocrine hormones. This differentiation occurs to a much greater extent when an EB formation step is included. Increased expression of endoderm markers during and after EB formation also correlated strongly with the size of cell clusters used to start EBs, as well as the addition of mesoderm- derived embryonic cells. This study demonstrates that a subset of differentiated HESC progeny adopt an endoderm fate and exhibit the capacity for further pancreatic lineage specification in vitro. Basal conditions were established for examining factors that can commit HESC-derived endoderm cells to specific pancreatic lineages. |
| تدمد: | 1557-7457 1536-2302 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ef6d682bda8eb70c6e1a5c38b6ff0b9Test https://doi.org/10.1089/clo.2006.8.96Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....1ef6d682bda8eb70c6e1a5c38b6ff0b9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15577457 15362302 |
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