Transmissible Plasmid ContainingSalmonella entericaHeidelberg Isolates Modulate Cytokine Production During Early Stage of Interaction with Intestinal Epithelial Cells
| العنوان: | Transmissible Plasmid ContainingSalmonella entericaHeidelberg Isolates Modulate Cytokine Production During Early Stage of Interaction with Intestinal Epithelial Cells |
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| المؤلفون: | Katherine Williams, Kuppan Gokulan, Steven L. Foley, Sangeeta Khare |
| المصدر: | DNA and Cell Biology. 35:443-453 |
| بيانات النشر: | Mary Ann Liebert Inc, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, medicine.medical_treatment, Gene Expression, Virulence, Cell Line, Microbiology, Mice, 03 medical and health sciences, Plasmid, Genetics, medicine, Animals, Humans, Macrophage, Secretion, Intestinal Mucosa, Molecular Biology, biology, Tumor Necrosis Factor-alpha, Macrophages, Interleukin-17, Granulocyte-Macrophage Colony-Stimulating Factor, Salmonella enterica, Interleukin, Epithelial Cells, Cell Biology, General Medicine, biology.organism_classification, Interleukin-10, 030104 developmental biology, Cytokine, Host-Pathogen Interactions, Tumor necrosis factor alpha, Chickens, Plasmids |
| الوصف: | The variation in cytokine production during bacterial invasion of human intestinal epithelial cells (IECs) is a contributing factor for progression of the infection. A few Salmonella enterica Heidelberg strains isolated from poultry products harbor transmissible plasmids (TPs), including those that encode a type-IV secretion system. Earlier, we showed that these TPs are responsible for increased virulence during infection. This study examines the potential role of these TPs in cytokine production in IECs. This study showed that S. Heidelberg strains containing TPs (we refer as virulent strains) caused decreased interleukin (IL)-10 production in IECs after 1 h infection. The virulent strains induced a high level of tumor necrosis factor-α production under identical conditions. The virulent strains of S. Heidelberg also altered the production of IL-2, IL-17, and granulocyte macrophage colony-stimulating factor compared to an avirulent strain. As a part of infection, bacteria cross the epithelial barrier and encounter intestinal macrophages. Hence, we examined the cytotoxic mechanism of strains of S. Heidelberg in macrophages. Scanning electron microscopy showed cell necrosis occurs during the early stage of infection. In conclusion, virulent S. Heidelberg strains were able to modify the host cytokine profile during the early stages of infection and also caused necrosis in macrophages. |
| تدمد: | 1557-7430 1044-5498 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::39c0c37ebf41e68668c5810ff0ea989cTest https://doi.org/10.1089/dna.2015.3142Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....39c0c37ebf41e68668c5810ff0ea989c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15577430 10445498 |
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