Xuhuai Goat H-FABP Gene Clone, Subcellular Localization of Expression Products and the Preparation of Transgenic Mice
| العنوان: | Xuhuai Goat H-FABP Gene Clone, Subcellular Localization of Expression Products and the Preparation of Transgenic Mice |
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| المؤلفون: | Wei Li, Caiye Zhu, Bi-chun Li, Yanhui Yin, Ya-ni Zhang, Guang-hui Wei, Wen-guang Cao, Li-xin Du |
| المصدر: | DNA and Cell Biology. 31:753-760 |
| بيانات النشر: | Mary Ann Liebert Inc, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Male, Signal peptide, Transcription, Genetic, Blotting, Western, Green Fluorescent Proteins, Mice, Transgenic, Biology, Fatty Acid-Binding Proteins, Real-Time Polymerase Chain Reaction, Green fluorescent protein, Mice, Complementary DNA, Testis, Genetics, Animals, Humans, Coding region, RNA, Messenger, Cloning, Molecular, Molecular Biology, Gene, Zebrafish, chemistry.chemical_classification, Reverse Transcriptase Polymerase Chain Reaction, Goats, Cell Biology, General Medicine, Subcellular localization, Molecular biology, Fusion protein, Rats, Amino acid, chemistry, Cattle, Female, lipids (amino acids, peptides, and proteins), Subcellular Fractions |
| الوصف: | The aim of this study was to clone the heart-type fatty acid binding protein (H-FABP) gene of Xuhuai goat, to explore it bioinformatically, and analyze the subcellular localization using enhanced green fluorescent protein (EGFP). The results showed that the coding sequence (CDS) length of Xuhuai goat H-FABP gene was 402 bp, encoding 133 amino acids (GenBank accession number AY466498.1). The H-FABP cDNA coding sequence was compared with the corresponding region of human, chicken, brown rat, cow, wild boar, donkey, and zebrafish. The similarity were 89%, 76%, 85%, 84%, 93%, 91%, 70%, respectively. For the corresponding amino acid sequences, the similarity were 90%, 79%, 88%, 97%, 95%, 94%, 72%, respectively. This study did not find the signal peptide region in the H-FABP protein; it revealed that H-FABP protein might be a nonsecreted protein. H-FABP expression was detected in vitro by reverse transcription-polymerase chain reaction (RT-PCR), and the EGFP-H-FABP fusion protein was localized to the cytoplasm. The gene could also be transiently and permanently expressed in mice. |
| تدمد: | 1557-7430 1044-5498 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26d95dd1dcbbb8ea391632f0a1b004dcTest https://doi.org/10.1089/dna.2011.1449Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....26d95dd1dcbbb8ea391632f0a1b004dc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15577430 10445498 |
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