Two Rainbow Trout (Oncorhynchus mykiss) Albumin Genes Are Differentially Regulated
| العنوان: | Two Rainbow Trout (Oncorhynchus mykiss) Albumin Genes Are Differentially Regulated |
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| المؤلفون: | Zhi Quan Gong, Choy L. Hew |
| المصدر: | DNA and Cell Biology. 17:207-216 |
| بيانات النشر: | Mary Ann Liebert Inc, 1998. |
| سنة النشر: | 1998 |
| مصطلحات موضوعية: | Fish Proteins, Untranslated region, DNA, Complementary, TATA box, Molecular Sequence Data, Biology, Albumins, Complementary DNA, Genetics, Animals, Coding region, Amino Acid Sequence, RNA, Messenger, Cloning, Molecular, Promoter Regions, Genetic, Molecular Biology, Gene, Messenger RNA, Base Sequence, cDNA library, Gene Expression Regulation, Developmental, Cell Biology, General Medicine, Molecular biology, Gene Expression Regulation, Liver, Oncorhynchus mykiss, Rainbow trout |
| الوصف: | Two distinct albumin cDNAs (rtALB1 and rtALB2) were isolated from the rainbow trout (Oncorhynchus mykiss) liver cDNA library. The rtALB1 cDNA (2761 bp) contains a 69 bp 5' untranslated region (UTR), a 1821 bp reading region, and a long 3' UTR of 872 bp. The rtALB2 cDNA (2250 bp) contains a 78 bp 5' UTR, a 1824 bp coding region, and a 348 bp 3' UTR. The two albumins are 81.5% and 77.5% identical in their nucleotides and protein sequences, respectively. Both rtALB1 and rtALB2 genes are expressed only in the liver. The albumin mRNA was first detected in 5-week-old embryos and was tissue-specific. The two albumin genes were differentially expressed, with the rtALB1 transcripts being 3 to 10 times more abundant than the rtALB2 transcripts. This differential expression was partially regulated at the transcriptional level. Promoter analysis showed that the rtALB1 gene had a typical albumin promoter structure. However, the rtALB2 promoter was abnormal in the TATA box region and was less effective in activating the reporter gene in the mammalian cell lines. These variations in rainbow trout albumin promoter sequences might account for their differences in transcriptional efficiency. |
| تدمد: | 1557-7430 1044-5498 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f28cd429419336102c3184ab98b5b2bTest https://doi.org/10.1089/dna.1998.17.207Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....9f28cd429419336102c3184ab98b5b2b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15577430 10445498 |
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