Muscarinic Receptor Activation Affects Pulmonary Artery Contractility in Sheep: The Impact of Maturation and Chronic Hypoxia on Endothelium-Dependent and Endothelium-Independent Function
| العنوان: | Muscarinic Receptor Activation Affects Pulmonary Artery Contractility in Sheep: The Impact of Maturation and Chronic Hypoxia on Endothelium-Dependent and Endothelium-Independent Function |
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| المؤلفون: | Joon Kim, Michael Giang, Ravi Goyal, Demosthenes G. Papamatheakis, Carla Blum Johnston, Alexander Brunnell, Quintin Blood, Dan Nguyen, Ricardo Paez, Lawrence D. Longo, Sean M. Wilson |
| المصدر: | High Altitude Medicine & Biology. 17:122-132 |
| بيانات النشر: | Mary Ann Liebert Inc, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Scientific Articles, medicine.medical_specialty, Endothelium, Physiology, Vasodilation, Muscarinic Antagonists, Pulmonary Artery, 030204 cardiovascular system & hematology, Biology, Bradykinin, Contractility, Phenylephrine, 03 medical and health sciences, 0302 clinical medicine, medicine.artery, Internal medicine, Muscarinic acetylcholine receptor, medicine, Animals, Hypoxia, Sheep, Electrical impedance myography, Altitude, Public Health, Environmental and Occupational Health, General Medicine, Hypoxia (medical), Receptors, Muscarinic, Acetylcholine, 030104 developmental biology, Endocrinology, medicine.anatomical_structure, Vasoconstriction, Pulmonary artery, cardiovascular system, Carbachol, Endothelium, Vascular, medicine.symptom |
| الوصف: | Giang, Michael, Demosthenes G. Papamatheakis, Dan Nguyen, Ricardo Paez, Carla Blum Johnston, Joon Kim, Alexander Brunnell, Quintin Blood, Ravi Goyal, Lawrence D. Longo, and Sean M. Wilson. Muscarinic receptor activation affects pulmonary artery contractility in sheep: the impact of maturation and chronic hypoxia on endothelium-dependent and endothelium-independent function. High Alt Med Biol. 17:122–132, 2015.—Muscarinic receptor activation in the pulmonary vasculature can cause endothelium-dependent vasodilation and smooth muscle-dependent vasoconstriction. Chronic hypoxia (CH) can modify both of these responses. This study aimed to assess the combined influence of CH and maturation on endothelium-dependent and endothelium-independent muscarinic-induced vasoreactivity. This was accomplished by performing wire myography on endothelium-intact or endothelium-disrupted pulmonary arterial rings isolated from normoxic or CH fetal and adult sheep. In endothelium-intact arteries, vasodilation was evaluated using cumulative bradykinin doses in phenylephrine and carbachol precontracted pulmonary arterial segments; and vasoconstriction was examined using cumulative doses of carbachol following bradykinin predilation. Effects of nonselective (atropine) and selective M1 (pirenzepine), M2 (AFDX116), and M3 (4-DAMP and Dau5884) muscarinic receptor antagonists were assessed in disrupted arteries. In normoxic arteries, bradykinin relaxation was twofold greater in the adult compared to fetus, while carbachol contraction was fourfold greater. In adult arteries, CH increased bradykinin relaxation and carbachol contraction. In vessels with intact endothelium, maturation and CH augmented maximal response and efficacy for carbachol constriction and bradykinin relaxation. Approximately 50%–80% of adult normoxic and CH endothelium-disrupted arteries contracted to acetylcholine, while ∼50% of fetal normoxic and ∼10% of CH arteries responded. Atropine reduced carbachol-induced contraction in all vessels. Adult normoxic vessels were most responsive to M3 antagonism, fetal to M2 antagonism, while M1 inhibition had no effect. Overall, muscarinic-induced pulmonary arterial contraction is partially endothelium dependent and appears to develop after birth. Fetuses are more reliant on M3 receptors while M2 receptors predominate in adults, whereas CH augments muscarinic-dependent pulmonary vasoconstriction in both. |
| تدمد: | 1557-8682 1527-0297 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29cbc7d3e55e8afcdecc31021857fde3Test https://doi.org/10.1089/ham.2015.0116Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....29cbc7d3e55e8afcdecc31021857fde3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15578682 15270297 |
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