دورية أكاديمية
Tyrosinase Nanoparticles: Understanding the Melanogenesis Pathway by Isolating the Products of Tyrosinase Enzymatic Reaction.
| العنوان: | Tyrosinase Nanoparticles: Understanding the Melanogenesis Pathway by Isolating the Products of Tyrosinase Enzymatic Reaction. |
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| المؤلفون: | Varghese, Paul K, Abu-Asab, Mones, Dimitriadis, Emilios K, Dolinska, Monika B, Morcos, George P, Sergeev, Yuri V |
| المصدر: | Department of Radiology & Diagnostic Medical Imaging |
| بيانات النشر: | LVHN Scholarly Works |
| سنة النشر: | 2021 |
| المجموعة: | Lehigh Valley Health Network: LVHN Scholarly Works |
| مصطلحات موضوعية: | Biosynthetic Pathways, Catalysis, Chemical Fractionation, Gene Expression, Melanins, Microscopy, Atomic Force, Monophenol Monooxygenase, Nanoparticles, Department of Radiology and Diagnostic Medical Imaging, Fellows and Residents, Diagnosis, Medicine and Health Sciences, Other Analytical, Diagnostic and Therapeutic Techniques and Equipment, Radiology |
| الوصف: | Human Tyrosinase (Tyr) is the rate-limiting enzyme of the melanogenesis pathway. Tyr catalyzes the oxidation of the substrate L-DOPA into dopachrome and melanin. Currently, the characterization of dopachrome-related products is difficult due to the absence of a simple way to partition dopachrome from protein fraction. Here, we immobilize catalytically pure recombinant human Tyr domain (residues 19-469) containing 6xHis tag to Ni-loaded magnetic beads (MB). Transmission electron microscopy revealed Tyr-MB were within limits of 168.2 ± 24.4 nm while the dark-brown melanin images showed single and polymerized melanin with a diameter of 121.4 ± 18.1 nm. Using Hill kinetics, we show that Tyr-MB has a catalytic activity similar to that of intact Tyr. The diphenol oxidase reactions of L-DOPA show an increase of dopachrome formation with the number of MB and with temperature. At 50 °C, Tyr-MB shows some residual catalytic activity suggesting that the immobilized Tyr has increased protein stability. In contrast, under 37 °C, the dopachrome product, which is isolated from Tyr-MB particles, shows that dopachrome has an orange-brown color that is different from the color of the mixture of L-DOPA, Tyr, and dopachrome. In the future, Tyr-MB could be used for large-scale productions of dopachrome and melanin-related products and finding a treatment for oculocutaneous albinism-inherited diseases. |
| نوع الوثيقة: | text |
| اللغة: | unknown |
| العلاقة: | https://scholarlyworks.lvhn.org/radiology-diagnostic-medical-imaging/303Test; https://pubmed.ncbi.nlm.nih.gov/33450959Test/ |
| الإتاحة: | https://scholarlyworks.lvhn.org/radiology-diagnostic-medical-imaging/303Test https://pubmed.ncbi.nlm.nih.gov/33450959Test/ |
| رقم الانضمام: | edsbas.7805866A |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |