دورية أكاديمية
Calcium sensitivity of vertebrate skeletal muscle myosin.
| العنوان: | Calcium sensitivity of vertebrate skeletal muscle myosin. |
|---|---|
| المؤلفون: | Pulliam, D L, Sawyna, V, Levine, R J |
| المصدر: | Department of Surgery |
| بيانات النشر: | LVHN Scholarly Works |
| سنة النشر: | 1983 |
| المجموعة: | Lehigh Valley Health Network: LVHN Scholarly Works |
| مصطلحات موضوعية: | Actins, Adenosine Triphosphatases, Animals, Calcium, Kinetics, Muscles, Myosin-Light-Chain Kinase, Myosins, Protein Kinases, Rabbits, Tropomyosin, Troponin, Department of Surgery, Medicine and Health Sciences |
| الوصف: | The calcium sensitivity of vertebrate skeletal muscle myosin has been investigated. Adenosinetriphosphatase (ATPase) activity was assayed in a reconstituted system composed of either purified rabbit myosin plus actin or myosin plus actin, tropomyosin, and troponin. The calcium sensitivity of actomyosin Mg-ATPase activity was found to be directly affected by the ionic strength of the assay medium. Actomyosin assayed at approximately physiological ionic strength (120 mM KCl) demonstrated calcium sensitivity which varied between 6 and 52%, depending on the myosin preparation and the age of the myosin. Mg-ATPase activity was increased when calcium was present in the assay medium at physiological ionic strength. Conversely, actomyosin Mg-ATPase activity assayed at a lower ionic strength (15 mM KCl) was inhibited by addition of calcium. Addition of tropomyosin and troponin to the assay increased the calcium sensitivity of the system at the physiological ionic strength still further (up to 99% calcium sensitivity) and conferred calcium sensitivity on the system at the lower ionic strength (greater than 90% calcium sensitivity). A correlation also existed between myosin's calcium sensitivity and the phosphorylated state of light chain 2. |
| نوع الوثيقة: | text |
| اللغة: | unknown |
| العلاقة: | https://scholarlyworks.lvhn.org/surgery/4297Test; https://pubmed.ncbi.nlm.nih.gov/6222760Test/ |
| الإتاحة: | https://scholarlyworks.lvhn.org/surgery/4297Test https://pubmed.ncbi.nlm.nih.gov/6222760Test/ |
| رقم الانضمام: | edsbas.58FDB7D1 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |