دورية أكاديمية
IL-7 mediated homeostatic expansion of human CD4+CD25+FOXP3+ regulatory T cells after depletion with anti-CD25 monoclonal antibody
| العنوان: | IL-7 mediated homeostatic expansion of human CD4+CD25+FOXP3+ regulatory T cells after depletion with anti-CD25 monoclonal antibody |
|---|---|
| المؤلفون: | Vignali, Debora, Gã¼rth, Clara Marie, Pellegrini, Silvia, Sordi, Valeria, Sizzano, Federico, Monti, Paolo, PIEMONTI, LORENZO |
| المساهمون: | Vignali, Debora, Gã¼rth, Clara Marie, Pellegrini, Silvia, Sordi, Valeria, Sizzano, Federico, Piemonti, Lorenzo, Monti, Paolo |
| بيانات النشر: | Lippincott Williams and Wilkins |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Adult, Allograft, Antibodies, Monoclonal, Cell Proliferation, Cells, Cultured, Diabetes Mellitus, Type 1, Dose-Response Relationship, Drug, Drug Therapy, Combination, Female, Forkhead Transcription Factor, Human, Immunologic Memory, Immunosuppressive Agent, Interleukin Receptor Common gamma Subunit, Interleukin-2 Receptor alpha Subunit, Interleukin-7, Interleukin-7 Receptor alpha Subunit, Lymphocyte Depletion, Male, Middle Aged, Mycophenolic Acid, Recombinant Fusion Protein, Signal Transduction, Sirolimu, T-Lymphocytes |
| الوصف: | The maintenance or expansion of regulatory T (Treg) cells has a fundamental role in the achievement of immunological tolerance after transplantation. Here we aimed to determine mechanisms of human Treg cell depletion and reconstitution after anti-CD25monoclonal antibody (mAb) treatment.Methods. Seventeen patients with type 1 diabetes who received pancreatic islet transplantation and anti-CD25 mAb as induction therapy were studied. Results.We observed an almost complete depletion of Treg cells after injection of anti-CD25 mAb. The kinetic of Treg cell depletion did not parallel the disappearance of CD25+ Tcells as CD25 is also rapidly downregulated and internalized. Regulatory Tcell reconstitution is completed within 6 months posttransplantation and appeared to be driven by IL-7-mediated homeostatic Tcell proliferation. Anti-CD25 mAb treatment sensitizes Treg cell to the biological effect of IL-7, possibly rendering more common γc-chain available to interact with CD127. Homeostatic Treg cell proliferation is resistant to the inhibitory effect of rapamycin and FK506 but can be blocked by the presence of mycophenolate mofetil. Conclusions. Our data suggest that a compensatory mechanism of IL-7-mediated homeostatic proliferation can restore the inhibitory network of Treg cell after anti-CD25 induction therapy in islet allotransplantation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/wos/WOS:000383912100022; volume:100; issue:9; firstpage:1853; lastpage:1861; numberofpages:9; journal:TRANSPLANTATION; http://hdl.handle.net/20.500.11768/60531Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84974779321; journals.lww.com/transplantjournal/pages/default.aspx |
| DOI: | 10.1097/TP.0000000000001276 |
| الإتاحة: | https://doi.org/20.500.11768/60531Test https://doi.org/10.1097/TP.0000000000001276Test https://hdl.handle.net/20.500.11768/60531Test |
| رقم الانضمام: | edsbas.2397F498 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1097/TP.0000000000001276 |
|---|