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مصطلحات موضوعية: F-Box-WD Repeat-Containing Protein 7, Ubiquitin-Protein Ligases, Computational biology, Biology, medicine.disease_cause, Precursor T-Cell Lymphoblastic Leukemia-Lymphoma, law.invention, Mice, law, Proto-Oncogene Proteins, microRNA, medicine, Animals, Genes, Tumor Suppressor, RNA, Small Interfering, Molecular Biology, Gene, Psychological repression, Genetics, Mutation, Bcl-2-Like Protein 11, Extra View, F-Box Proteins, PTEN Phosphohydrolase, Cancer, Membrane Proteins, Cell Biology, Oncomir, medicine.disease, Gene Expression Regulation, Neoplastic, Disease Models, Animal, MicroRNAs, Gene Knockdown Techniques, Suppressor, Apoptosis Regulatory Proteins, Developmental Biology, Genetic screen
الوصف: Individual microRNAs (miRNAs) have been implicated as oncogenes in experimental cancer models and their expression may affect clinical outcomes. To gain a more comprehensive view of miRNA action in leukemia, we analyzed miRNA expression patters in T-cell leukemia ALL (T-ALL) and cross-referenced the results with an unbiased genetic screen and computational analyses.1 We found that multiple microRNAs contribute to leukmogenesis and act as multi-targeted regulators of several tumor suppressor genes. The oncomirs form a network of overlapping and partially redundant interactions that stabilize the malignant phenotype though coordinate repression of cellular failsafe programs. The emerging network pattern of oncomir action is distinct from the notion of single oncogenic 'driver' mutation. We will discuss experimental, diagnostic and therapeutic implications of this concept of miRNA action in cancer.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc75e5c5d63d4c5505bc9145990987f7Test
https://europepmc.org/articles/PMC3218598Test/ -
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المؤلفون: Nando Nakazawa, Zenya Naito, Yoko Matsuda, Murray Korc, Kiyoko Kawahara, Toshiyuki Ishiwata
مصطلحات موضوعية: Cancer Research, Mice, Nude, Nerve Tissue Proteins, macromolecular substances, Biology, Polymerase Chain Reaction, Small hairpin RNA, Nestin, Mice, Intermediate Filament Proteins, Cell Movement, Tubulin, Cell Line, Tumor, Pancreatic cancer, Cell Adhesion, medicine, Animals, Humans, Regeneration, Neoplasm Invasiveness, RNA, Messenger, Neoplasm Metastasis, RNA, Small Interfering, Cell adhesion, Cell Proliferation, Pharmacology, Mice, Inbred BALB C, Cell growth, Cadherin, Cell adhesion molecule, Stem Cells, Cell Differentiation, Cadherins, medicine.disease, Actin cytoskeleton, Actins, Pancreatic Neoplasms, Actin Cytoskeleton, Oncology, Gene Knockdown Techniques, embryonic structures, Commentary, Cancer research, Molecular Medicine, Research Paper, Carcinoma, Pancreatic Ductal
الوصف: Nestin, is a class VI intermediate filament (IF) that is expressed in 30% of pancreatic ductal adenocarcinoma (PDAC) cases, and its expression in PDAC positively correlates with peripancreatic invasion. An expression vector carrying a short hairpin RNA (shRNA) targeting nestin was stably transfected into PANC-1 and PK-45H human pancreatic cancer cells, which express high nestin levels. Alterations in morphology and alignment of actin filaments and α-tubulin were examined by phase-contrast and immunocytochemistry. Effects on cell growth, migration in scratch and Boyden chamber assays, invasion, cell adhesion, and in vivo growth were determined. Differences in mRNA levels were examined by arrays. Nestin shRNA-transfected cells exhibited decreased nestin expression, a sheet-like appearance with tight cell-cell adhesion, increased expression of filamentous F-actin and E-cadherin, and attenuated migration and invasion, both of which were enhanced following nestin re-expression. Expression of α-tubulin, and in vitro cell growth and adhesion were not altered by nestin down-regulation, whereas hepatic metastases were decreased. Thus, nestin plays important roles in pancreatic cancer cell migration, invasion and metastasis by selectively modulating the expression of actin and cell adhesion molecules, and may therefore be a novel therapeutic target in PDAC.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f03ed02724643a388ec2ec326407d652Test
https://europepmc.org/articles/PMC3230315Test/
