Glutamic Acid Decarboxylase Autoantibody Detection by Electrochemiluminescence Assay Identifies Latent Autoimmune Diabetes in Adults with Poor Islet Function
| العنوان: | Glutamic Acid Decarboxylase Autoantibody Detection by Electrochemiluminescence Assay Identifies Latent Autoimmune Diabetes in Adults with Poor Islet Function |
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| المؤلفون: | Zhiguang Zhou, Yu Liu, Gan Huang, Qing Liu, Jing Zou, Li Qian, Ying Zhou, Yuxiao Zhu, Tao Yang |
| المصدر: | Diabetes & Metabolism Journal, Vol 44, Iss 2, Pp 260-266 (2020) Diabetes & Metabolism Journal |
| بيانات النشر: | Korean Diabetes Association, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Adult, Male, endocrine system, Adolescent, endocrine system diseases, autoantibodies, Endocrinology, Diabetes and Metabolism, Glutamate decarboxylase, 030209 endocrinology & metabolism, 030204 cardiovascular system & hematology, c-peptide, lcsh:Diseases of the endocrine glands. Clinical endocrinology, Islets of Langerhans, Young Adult, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Diabetes mellitus, medicine, Humans, latent autoimmune diabetes in adults, Technology/Devise, Type 1 diabetes, lcsh:RC648-665, biology, business.industry, C-peptide, Autoantibody, Type 2 Diabetes Mellitus, nutritional and metabolic diseases, Middle Aged, medicine.disease, Diabetes Mellitus, Type 1, Phenotype, Diabetes Mellitus, Type 2, chemistry, Case-Control Studies, Immunology, biology.protein, glutamate decarboxylase, Female, Original Article, Radiobinding assay, Antibody, business |
| الوصف: | Background: The detection of glutamic acid decarboxylase 65 (GAD65) autoantibodies is essential for the prediction and diag nosis of latent autoimmune diabetes in adults (LADA). The aim of the current study was to compare a newly developed electro chemiluminescence (ECL)-GAD65 antibody assay with the established radiobinding assay, and to explore whether the new assay could be used to define LADA more precisely. Methods: Serum samples were harvested from 141 patients with LADA, 95 with type 1 diabetes mellitus, and 99 with type 2 dia betes mellitus, and tested for GAD65 autoantibodies using both the radiobinding assay and ECL assay. A glutamic acid decarbox ylase antibodies (GADA) competition assay was also performed to assess antibody affinity. Furthermore, the clinical features of these patients were compared. Results: Eighty-eight out of 141 serum samples (62.4%) from LADA patients were GAD65 antibody-positive by ECL assay. Com pared with ECL-GAD65 antibody-negative patients, ECL-GAD65 antibody-positive patients were leaner (P |
| اللغة: | English |
| تدمد: | 2233-6087 2233-6079 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb2d9264896b26a9cda74d22d4eb90f4Test http://e-dmj.org/upload/pdf/dmj-44-260.pdfTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....cb2d9264896b26a9cda74d22d4eb90f4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22336087 22336079 |
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