Purification of heat-labile enterotoxin from an enterotoxin from an enterotoxigenic Escherichia coli of human origin by monoclonal immunoaffinity chromatography
| العنوان: | Purification of heat-labile enterotoxin from an enterotoxin from an enterotoxigenic Escherichia coli of human origin by monoclonal immunoaffinity chromatography |
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| المؤلفون: | Kwi-Won Park, Ho Kim, K H Rhee, H K Chung, Maeng Je Cho, J S Kang, Chang Yong Cha, W H Chang, Ik-Sang Kim, Myung-Sik Choi |
| المصدر: | Journal of Korean Medical Science |
| بيانات النشر: | Korean Academy of Medical Sciences, 1987. |
| سنة النشر: | 1987 |
| مصطلحات موضوعية: | Enzyme-Linked Immunosorbent Assay, Enterotoxin, Heat-labile enterotoxin, medicine.disease_cause, Chromatography, Affinity, Enterotoxins, Mice, Affinity chromatography, Enterotoxigenic Escherichia coli, Escherichia coli, medicine, Animals, Humans, Polyacrylamide gel electrophoresis, Mice, Inbred BALB C, biology, Antibodies, Monoclonal, General Medicine, biology.organism_classification, Molecular biology, Monoclonal, Chromatography, Gel, Electrophoresis, Polyacrylamide Gel, Rabbits, Bacteria, Research Article |
| الوصف: | Heat-labile enterotoxin (LT) was purified from an enterotoxigenic Escherichia coli 015H11 of human origin. The purification steps included French pressure cell disruption of the bacteria, salting-out, DEAE-Sephacel on chromatography. Application of this procedure resulted in a 95.1-fold purification of LT with a yield of 19.9% as determined by rabbit ileal loop assay. The final LT preparation showed only one protein-staining band on polyacrylamide gel electrophoresis, indicating that the purified LT was homogeneous. |
| تدمد: | 1598-6357 1011-8934 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db1f25d62fc3a370471aeacd7199a851Test https://doi.org/10.3346/jkms.1987.2.1.65Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....db1f25d62fc3a370471aeacd7199a851 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15986357 10118934 |
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