دورية أكاديمية
Diagnosis of Neoplasia in Barrett’s Esophagus using Vital-dye Enhanced Fluorescence Imaging
| العنوان: | Diagnosis of Neoplasia in Barrett’s Esophagus using Vital-dye Enhanced Fluorescence Imaging |
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| المؤلفون: | Perl, Daniel P., Parikh, Neil, Chang, Shannon, Peng, Paul, Thekkek, Nadhi, Lee, Michelle H., Polydorides, Alexandros D., Mitcham, Josephine, Richards-Kortum, Rebecca, Anandasabapathy, Sharmila |
| بيانات النشر: | JoVE |
| سنة النشر: | 2014 |
| المجموعة: | Rice University: Digital Scholarship Archive |
| الوصف: | The ability to differentiate benign metaplasia in Barrett’s Esophagus (BE) from neoplasia in vivo remains difficult as both tissue types can be flat and indistinguishable with white light imaging alone. As a result, a modality that highlights glandular architecture would be useful to discriminate neoplasia from benign epithelium in the distal esophagus. VFI is a novel technique that uses an exogenous topical fluorescent contrast agent to delineate high grade dysplasia and cancer from benign epithelium. Specifically, the fluorescent images provide spatial resolution of 50 to 100 μm and a field of view up to 2.5 cm, allowing endoscopists to visualize glandular morphology. Upon excitation, classic Barrett’s metaplasia appears as continuous, evenly-spaced glands and an overall homogenous morphology; in contrast, neoplastic tissue appears crowded with complete obliteration of the glandular framework. Here we provide an overview of the instrumentation and enumerate the protocol of this new technique. While VFI affords a gastroenterologist with the glandular architecture of suspicious tissue, cellular dysplasia cannot be resolved with this modality. As such, one cannot morphologically distinguish Barrett’s metaplasia from BE with Low-Grade Dysplasia via this imaging modality. By trading off a decrease in resolution with a greater field of view, this imaging system can be used at the very least as a red-flag imaging device to target and biopsy suspicious lesions; yet, if the accuracy measures are promising, VFI may become the standard imaging technique for the diagnosis of neoplasia (defined as either high grade dysplasia or cancer) in the distal esophagus. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | Perl, Daniel P., Parikh, Neil, Chang, Shannon, et al. "Diagnosis of Neoplasia in Barrett’s Esophagus using Vital-dye Enhanced Fluorescence Imaging." Journal of Visualized Experiments, 87, (2014) JoVE: https://doi.org/10.3791/50992Test.; https://hdl.handle.net/1911/94800Test; https://doi.org/10.3791/50992Test |
| DOI: | 10.3791/50992 |
| الإتاحة: | https://doi.org/10.3791/50992Test https://hdl.handle.net/1911/94800Test |
| حقوق: | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. |
| رقم الانضمام: | edsbas.47C73450 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3791/50992 |
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