Synergistic Effects of the Combination of 20-Hydroxyecdysone with Ampicillin and Gentamicin Against Methicillin-Resistant Staphylococcus aureus
| العنوان: | Synergistic Effects of the Combination of 20-Hydroxyecdysone with Ampicillin and Gentamicin Against Methicillin-Resistant Staphylococcus aureus |
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| المؤلفون: | Jung-Hoon Kim, Jin-Kyung Kim, Sang Heon Lee, Hong-Seob So, Eun Sook Kim, Rae Kil Park, Seung-Il Jeong, Channy Park, Kang-Min Lee, Shin-Moon Kim |
| المصدر: | Journal of Microbiology and Biotechnology. 19:1576-1581 |
| بيانات النشر: | Journal of Microbiology and Biotechnology, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Methicillin-Resistant Staphylococcus aureus, Time Factors, Meticillin, Microbial Sensitivity Tests, Biology, medicine.disease_cause, Staphylococcal infections, Plant Roots, Applied Microbiology and Biotechnology, Microbiology, Ampicillin, medicine, Humans, Achyranthes, Plant Extracts, Achyranthes japonica, Drug Synergism, General Medicine, Staphylococcal Infections, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, medicine.disease, Methicillin-resistant Staphylococcus aureus, Anti-Bacterial Agents, Ecdysterone, Staphylococcus aureus, Drug Therapy, Combination, Gentamicin, Gentamicins, Antibacterial activity, Phytotherapy, Biotechnology, medicine.drug |
| الوصف: | The emergence of methicillin-resistant of Staphylococcus aureus (MRSA) has led to an urgent need for the discovery and development of new antibacterial agents. As part of an ongoing investigation into the antibacterial properties of the natural products, 20-Hydroxyecdysone (20E), isolated from the roots of Achyranthes japonica Nakai, was found to be active methicillin-resistant Staphylococcus aureus (MRSA), either alone or in combination with ampicillin (AM) or gentamicin (GM), vis checkerboard assay. This study investigated the antibacterial activity of 20E, which exhibited poor antibacterial activity (MIC=250-500 microg/mL) against all the bacterial strains tested. The combined activity of ampicillin (AM), gentamicin (GE) plus 20E against MRSA resulted in fractional inhibitory concentrations (FICs) ranging from 4.00 to 0.031 microg/mL, respectively. Meanwhile, the FIC index ranged from 0.16-4.50, indicating a marked synergistic relationship between AM, GE and 20E against MRSA with enterotoxin gene. Time-kill assays also showed a decrease remarkably between the combination and the more active compound. Therefore, this study demonstrated that AM, GE, and 20E can act synergistically in inhibiting MRSA in vitro. |
| تدمد: | 1738-8872 1017-7825 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::939982be532699bb29cfdf97848a7586Test https://doi.org/10.4014/jmb.0903.03015Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....939982be532699bb29cfdf97848a7586 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17388872 10177825 |
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