Performance of Homologous and Heterologous Prime-Boost Immunization Regimens of Recombinant Adenovirus and Modified Vaccinia Virus Ankara Expressing an Ag85B-TB10.4 Fusion Protein against Mycobacterium tuberculosis
| العنوان: | Performance of Homologous and Heterologous Prime-Boost Immunization Regimens of Recombinant Adenovirus and Modified Vaccinia Virus Ankara Expressing an Ag85B-TB10.4 Fusion Protein against Mycobacterium tuberculosis |
|---|---|
| المؤلفون: | Yiming Kou, Bo Sun, Linjun Cai, Chunlai Jiang, Zhilei Zhao, Feng Gao, Mingming Wan, Wei Shi, Yongqing Xu, Jie Liu, Wei Wei |
| المصدر: | Journal of Microbiology and Biotechnology. 28:1022-1029 |
| بيانات النشر: | Journal of Microbiology and Biotechnology, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | biology, business.industry, Heterologous, 02 engineering and technology, General Medicine, 021001 nanoscience & nanotechnology, 030226 pharmacology & pharmacy, Applied Microbiology and Biotechnology, Virology, Virus, Viral vector, 03 medical and health sciences, Regimen, chemistry.chemical_compound, 0302 clinical medicine, Immune system, chemistry, Immunization, biology.protein, Medicine, Vaccinia, Antibody, 0210 nano-technology, business, Biotechnology |
| الوصف: | Tuberculosis (TB) remains a serious health issue around the word. Adenovirus (Ad)-based vaccine and modified vaccinia virus Ankara (MVA)-based vaccine have emerged as two of the most promising immunization candidates over the past few years. However, the performance of the homologous and heterologous prime-boost immunization regimens of these two viral vector-based vaccines remains unclear. In the present study, we constructed recombinant Ad and MVA expressing an Ag85B-TB10.4 fusion protein (AdH4 and MVAH4) and evaluated the impact of their different immunization regimens on the humoral and cellular immune responses. We found that the viral vector-based vaccines could generate significantly higher levels of antigen-specific antibodies, IFN-γ-producing splenocytes, CD69⁺CD8⁺ T cells, and IFN-γ secretion when compared with bacillus Calmette-Guerin (BCG) in a mouse model. AdH4-containing immunization regimens (AdH4-AdH4, AdH4-MVAH4, and MVAH4-AdH4) induced significantly stronger antibody responses, much more IFN-γ-producing splenocytes and CD69⁺CD8⁺ T cells, and higher levels of IFN-γ secretion when compared with the MVAH4-MVAH4 immunization regimen. The number of IFN-γ-producing splenocytes sensitive to CD8⁺ T-cell restricted peptides of Ag85B (9-1p and 9-2p) and Th1-related cytokines (IFN-γ and TNF-α) in the AdH4-MVAH4 heterologous prime-boost regimen immunization group was significantly higher than that in the other viral vector-based vaccine- and BCG-immunized groups, respectively. These results indicate that an immunization regimen involving AdH4 may have a higher capacity to induce humoral and cellular immune responses against TB in mice than that by regimens containing BCG or MVAH4 alone, and the AdH4-MVAH4 prime-boost regimen may generate an ideal protective effect. |
| تدمد: | 1738-8872 1017-7825 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::d3acbbddaa8abe69321ea74c31ed487cTest https://doi.org/10.4014/jmb.1712.12064Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........d3acbbddaa8abe69321ea74c31ed487c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17388872 10177825 |
|---|