دورية أكاديمية
Comparison of zonal elution and nonlinear chromatography in determination of the interaction between seven drugs and immobilised beta(2)-adrenoceptor
| العنوان: | Comparison of zonal elution and nonlinear chromatography in determination of the interaction between seven drugs and immobilised beta(2)-adrenoceptor |
|---|---|
| المؤلفون: | Li, Qian, Wang, Jing, Zheng, Yuqing Yuan, Yang, Lingjian, Zhang, Yajun, Bian, Liujiao, Zheng, Jianbin, Li, Zijian, Zhao, Xinfeng, Zhang, Youyi |
| المساهمون: | Zhao, XF (reprint author), NW Univ Xian, Coll Life Sci, Minist Educ, Key Lab Resource Biol & Biotechnol Western China, Xian 710069, Peoples R China., NW Univ Xian, Coll Life Sci, Minist Educ, Key Lab Resource Biol & Biotechnol Western China, Xian 710069, Peoples R China., Yanan Univ, Xian Innovat Coll, Xian 710100, Peoples R China., Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100083, Peoples R China., Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China. |
| المصدر: | SCI ; PubMed ; EI |
| بيانات النشر: | JOURNAL OF CHROMATOGRAPHY A |
| سنة النشر: | 2015 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | Affinity chromatography, Zonal elution, Nonlinear chromatography, Drug-protein interaction, beta(2)-Adrenoceptor, PERFORMANCE AFFINITY-CHROMATOGRAPHY, HUMAN SERUM-ALBUMIN, SMALL MOLECULE INTERACTIONS, SURFACE-PLASMON RESONANCE, PROTEIN-COUPLED RECEPTOR, LIGAND BINDING-AFFINITY, LIQUID-CHROMATOGRAPHY, NONCOMPETITIVE INHIBITORS, CONFORMATIONAL MOBILITY, STATIONARY PHASES |
| الوصف: | Zonal elution and nonlinear chromatography are two mainstream models for the determination of drug-protein interaction in affinity chromatography. This work intended to compare the results by zonal elution with that by nonlinear chromatography when it comes to the analysis of the interaction between seven drugs and immobilised beta(2)-adrenoceptor (beta(2)-AR). The results of the zonal elution showed that clorprenaline, clenbuterol, methoxyphenamine, salbutamol, terbutaline, tulobuterol and bambuterol have only one type of binding site on immobilised beta(2)-AR, while nonlinear chromatography confirmed the existence of at least two types of binding sites between beta(2)-AR and clorprenaline, clenbuterol and bambuterol. On these sites, both zonal elution and nonlinear chromatography presented the same rank order for the association constants of the seven drugs. Compared with the data from zonal elution, the association constants calculated using nonlinear chromatography gave a good linear response to the corresponding values by radio-ligand binding assay. The sampling efficiencies of nonlinear chromatography were clearly higher than zonal elution. Nonlinear chromatography will probably become a powerful alternative for the high throughput determination of drug-protein interaction. (C) 2015 Elsevier B.V. All rights reserved. ; National Natural Science Foundation of China [21475103]; Program for Changjiang Scholars and Innovative Research Team in University [IRT1174]; Project for Innovative Research Team of Research and Technology of Shaanxi Province [2013KCT-24]; National Key Scientific Instrument and Equipment Development Project of China [2013YQ170525, 2013YQ17052509] ; SCI(E) ; EI ; PubMed ; ARTICLE ; zhaoxf@nwu.edu.cn; zhangyy@bjmu.edu.cn ; 75-83 ; 1401 |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | English |
| تدمد: | 0021-9673 1873-3778 |
| العلاقة: | JOURNAL OF CHROMATOGRAPHY A.2015,1401,75-83.; 1299553; http://hdl.handle.net/20.500.11897/418615Test; WOS:000356550800010 |
| DOI: | 10.1016/j.chroma.2015.05.012 |
| الإتاحة: | https://doi.org/20.500.11897/418615Test https://doi.org/10.1016/j.chroma.2015.05.012Test https://hdl.handle.net/20.500.11897/418615Test |
| رقم الانضمام: | edsbas.7959F8C8 |
| قاعدة البيانات: | BASE |
| تدمد: | 00219673 18733778 |
|---|---|
| DOI: | 10.1016/j.chroma.2015.05.012 |