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Multifaceted modes of γ-tubulin complex recruitment and microtubule nucleation at mitotic centrosomes

التفاصيل البيبلوغرافية
العنوان: Multifaceted modes of γ-tubulin complex recruitment and microtubule nucleation at mitotic centrosomes
المؤلفون: Zhu, Zihan, Becam, Isabelle, Tovey, Corinne A, Elfarkouchi, Abir, Yen, Eugenie C, Bernard, Fred, Guichet, Antoine, Conduit, Paul T
المصدر: nlmid: 0375356 ; essn: 1540-8140
بيانات النشر: Rockefeller University Press
//dx.doi.org/10.1083/jcb.202212043
Journal of Cell Biology
سنة النشر: 2023
المجموعة: Apollo - University of Cambridge Repository
مصطلحات موضوعية: Microtubule-Organizing Center, Centrosome, Microtubules, Cytosol, Animals, Drosophila, Tubulin, Cytoskeletal Proteins, Homeodomain Proteins, Drosophila Proteins
الوصف: Funder: Centre National de la Recherche Scientifique ; Funder: Fondation Bettencourt Schueller ; Microtubule nucleation is mediated by γ-tubulin ring complexes (γ-TuRCs). In most eukaryotes, a GCP4/5/4/6 “core” complex promotes γ-tubulin small complex (γ-TuSC) association to generate cytosolic γ-TuRCs. Unlike γ-TuSCs, however, this core complex is non-essential in various species and absent from budding yeasts. In Drosophila, Spindle defective-2 (Spd-2) and Centrosomin (Cnn) redundantly recruit γ-tubulin complexes to mitotic centrosomes. Here, we show that Spd-2 recruits γ-TuRCs formed via the GCP4/5/4/6 core, but Cnn can recruit γ-TuSCs directly via its well-conserved CM1 domain, similar to its homologs in budding yeast. When centrosomes fail to recruit γ-tubulin complexes, they still nucleate microtubules via the TOG domain protein Mini-spindles (Msps), but these microtubules have different dynamic properties. Our data, therefore, help explain the dispensability of the GCP4/5/4/6 core and highlight the robustness of centrosomes as microtubule organizing centers. They also suggest that the dynamic properties of microtubules are influenced by how they are nucleated.
نوع الوثيقة: article in journal/newspaper
وصف الملف: application/pdf
اللغة: English
العلاقة: https://www.repository.cam.ac.uk/handle/1810/357319Test
الإتاحة: https://www.repository.cam.ac.uk/handle/1810/357319Test
حقوق: Attribution 4.0 International ; https://creativecommons.org/licenses/by/4.0Test/
رقم الانضمام: edsbas.EC8F391C
قاعدة البيانات: BASE
الوصف
الوصف غير متاح.