دورية أكاديمية
Dynamics of Vaginal Microbiota during Estrous Cycle and its Association with Reproductive Hormones in Bubalus bubalis
| العنوان: | Dynamics of Vaginal Microbiota during Estrous Cycle and its Association with Reproductive Hormones in Bubalus bubalis |
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| المؤلفون: | P., Mahesh, Suthar, Vishal S., Patil, Deepak B., Bagatharia, Snehal B., Joshi, Madhavi, Joshi, Chaitanya G. |
| المصدر: | THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY; Vol. 17 No. 03 (2021): INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY; 11-18 ; 2395-1176 ; 2394-0247 |
| بيانات النشر: | Journal of Applied Pharmaceutical Sciences and Research |
| سنة النشر: | 2021 |
| المجموعة: | The Indian Journal of Veterinary Science & Biotechnology (IJVSBT) |
| مصطلحات موضوعية: | Bubalus bubalis, Estrous cycle, Reproductive hormones, Vaginal microbiota |
| الوصف: | This experiment aimed to evaluate the dynamics of vaginal microbiota during different estrous cycle stages in cyclic buffalo heifers using metagenomic analysis and its association with estradiol (E2) and progesterone (P4) hormones. Out of nine buffalo heifers, eight responded to ovsynch protocol were included in the trial. Vaginal flushing was carried out using the assembly of sterile normal saline with a syringe and AI sheath on day 0 (estrus), day 4 (metestrus), day 9 (diestrus), and day 18 (proestrus) of the estrous cycle followed by blood sample collection. The vaginal flushing was lyophilized, DNA were isolated and amplified with Phusion primer for bacteria (16S rRNA), fungus (ITS 6-8), and archaea (16S rRNA). The amplicon library was constructed, sequenced, and annotated through MG-RAST server with RNA database SILVA ssu with 98% identity. The results of the study revealed four major domain- bacteria (93.8%), eukaryota (5.7%), archaea 0.006% and unclassified sequences (0.41%). A total of 2196 bacterial species, 17 fungal and two archaeal species, were detected, confirming the rich diversity of vaginal microflora in buffalo heifers. The highly abundant domain of bacteria contained 18 bacterial phyla, which were grouped into four high ( |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://ijvsbt.org/index.php/journal/article/view/1520/1086Test; https://ijvsbt.org/index.php/journal/article/view/1520Test |
| DOI: | 10.21887/ijvsbt.17.3.3 |
| الإتاحة: | https://doi.org/10.21887/ijvsbt.17.3.3Test https://ijvsbt.org/index.php/journal/article/view/1520Test |
| حقوق: | Copyright (c) 2021 The Indian Journal of Veterinary Sciences and Biotechnology ; http://creativecommons.org/licenses/by-nc-sa/4.0Test |
| رقم الانضمام: | edsbas.C25B4877 |
| قاعدة البيانات: | BASE |
| DOI: | 10.21887/ijvsbt.17.3.3 |
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