Circular RNA circFOXO3 promotes prostate cancer progression through sponging miR‐29a‐3p
| العنوان: | Circular RNA circFOXO3 promotes prostate cancer progression through sponging miR‐29a‐3p |
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| المؤلفون: | Peiqing Zhao, Yali Lu, Xuechao Wan, Yan Huang, Yajuan Liu, Xinxin Xiang, Liang Li, Yingyi Zhang, Zhe Kong, Yi Xu, Yao Li |
| المصدر: | Journal of Cellular and Molecular Medicine |
| بيانات النشر: | John Wiley and Sons Inc., 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, miRNA sponge, Apoptosis, Biology, medicine.disease_cause, urologic and male genital diseases, miR‐29a‐3p, 03 medical and health sciences, Exon, Prostate cancer, 0302 clinical medicine, Circular RNA, medicine, Biomarkers, Tumor, Tumor Cells, Cultured, Humans, prostate cancer (PCa), Aged, Cell Proliferation, Forkhead Box Protein O3, RNA, circFOXO3, Prostatic Neoplasms, Cell Biology, Original Articles, RNA, Circular, Cell cycle, medicine.disease, Prognosis, Gene Expression Regulation, Neoplastic, MicroRNAs, 030104 developmental biology, 030220 oncology & carcinogenesis, FOXO3, Cancer research, Molecular Medicine, biomarker, Original Article, Carcinogenesis |
| الوصف: | Circular RNA FOXO3 (CircFOXO3, also termed as Hsa_circ_0006404) is derived from exon 2 of forkhead box O3 (FOXO3) gene, and abnormal expression is shown in different diseases. However, whether circFOXO3 plays important roles in tumorigenesis and progression of prostate cancer (PCa) remains unclear. In this study, we found that circFOXO3 was up‐regulated in both PCa tissues and serum samples. Moreover, circFOXO3 was positively correlated with the Gleason score in PCa samples. CircFOXO3 was observed to be up‐regulated in Gleason score > 6 PCa samples compared with Gleason score = 6 PCa samples. Knock‐down circFOXO3 could remarkably inhibit PCa cell cycle, proliferation and promote cell apoptosis in vitro. Furthermore, we demonstrated circFOXO3 could act as miR‐29a‐3p sponge to up‐regulate SLC25A15 expression by bioinformatics analysis, dual‐luciferase reporter assays and biotinylated RNA pull‐down assays. SLC25A15 could reverse the tumour suppressing roles of knock‐down circFOXO3 in PCa. Of note, we found that miR‐29a‐3p was down‐regulated; however, SLC25A15 was overexpressed in PCa samples compared with normal tissues. In conclusion, circFOXO3 acts as a miR‐29a‐3p sponge to exhibit oncogenic activity that affects the cell cycle and cell apoptosis in PCa through transcriptional up‐regulation of SLC25A15. Our analysis suggests circFOXO3 could act as promising prostate cancer biomarkers. |
| اللغة: | English |
| تدمد: | 1582-4934 1582-1838 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f86b759de96fe2f944a82ea57a673d56Test http://europepmc.org/articles/PMC6933405Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f86b759de96fe2f944a82ea57a673d56 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15824934 15821838 |
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