miR‐1, miR‐499 and miR‐208 are sensitive markers to diagnose sudden death due to early acute myocardial infarction
| العنوان: | miR‐1, miR‐499 and miR‐208 are sensitive markers to diagnose sudden death due to early acute myocardial infarction |
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| المؤلفون: | Enrica Pinchi, Alessandro Santurro, Luigi Cipolloni, Paola Frati, Margherita Neri, Matteo Scopetti, Rocco Valerio Viola, Raffaele La Russa, Mariarosaria Aromatario, Vittorio Fineschi, Matteo Fabbri, Emanuela Turillazzi, Aniello Maiese |
| المصدر: | Journal of Cellular and Molecular Medicine |
| بيانات النشر: | John Wiley and Sons Inc., 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Oncology, Adult, Genetic Markers, Male, medicine.medical_specialty, Myocardial Infarction, Socio-culturale, acute myocardial infarction, Tryptase, Disease, miR-499, miR‐1, miR‐499, Real-Time Polymerase Chain Reaction, Sudden death, sudden cardiac death, Sudden cardiac death, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, Troponin I, medicine, Humans, Myocardial infarction, cardiovascular diseases, Pathological, Aged, biology, business.industry, miR-208, Cell Biology, Original Articles, miR‐208, Middle Aged, medicine.disease, miR-1, MicroRNAs, 030104 developmental biology, Death, Sudden, Cardiac, 030220 oncology & carcinogenesis, biology.protein, Molecular Medicine, Original Article, Female, Differential diagnosis, business |
| الوصف: | MicroRNAs (miRNAs) are strongly up‐regulated under pathological stress and in a wide range of diseases. In recent years, miRNAs are under investigation for their potential use as biomarkers in cardiovascular diseases. We investigate whether specific cardio‐miRNAs are overexpressed in heart samples from subjects deceased for acute myocardial infarction (AMI) or sudden cardiac death (SCD), and whether miRNA could help differentiate between them. Forty four cases of death due to cardiovascular disease were selected, respectively, 19 cases categorized as AMI and 25 as SCD. Eighteen cases of traumatic death without pathological cardiac involvement were selected as control. Immunohistochemical investigation was performed for CD15, IL‐15, Cx43, MCP‐1, tryptase, troponin C and troponin I. Reverse transcription and quantitative real‐time PCR were performed for miR‐1, miR‐133, miR‐208 and miR‐499. In AMI group, stronger immunoreaction for the CD15, IL‐15 and MCP‐1 antibodies was detectable compared with SCD and control. Cx43 showed a negative reaction with respect to the other groups. Real‐time PCR results showed a down‐regulation of all miRNAs in the AMI group compared with SCD and control. The selected miRNAs presented high accuracy in discriminating SCD from AMI (miR‐1 and miR‐499) and AMI from control (miR‐208) representing a potential aid for both clinicians and pathologists for differential diagnosis. |
| اللغة: | English |
| تدمد: | 1582-4934 1582-1838 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c6b8323ec5016080ac5d29a92a1b4ccTest http://europepmc.org/articles/PMC6714215Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7c6b8323ec5016080ac5d29a92a1b4cc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15824934 15821838 |
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