Loss of wbpL disrupts O‐polysaccharide synthesis and impairs virulence of plant‐associated Pseudomonas strains
| العنوان: | Loss of wbpL disrupts O‐polysaccharide synthesis and impairs virulence of plant‐associated Pseudomonas strains |
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| المؤلفون: | Kutschera, Alexander, Schombel, Ursula, Wröbel, Michelle, Gisch, Nicolas, Ranf, Stefanie |
| المصدر: | Molecular Plant Pathology |
| بيانات النشر: | John Wiley and Sons Inc., 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Virulence, fungi, lipopolysaccharide, Arabidopsis, food and beverages, Pseudomonas syringae, Original Articles, Gene Knockout Techniques, motility, Bacterial Proteins, Solanum lycopersicum, Polysaccharides, Multigene Family, Pseudomonas, Original Article, O‐polysaccharide, Plant Diseases |
| الوصف: | Summary Despite its importance for membrane stability and pathogenicity of mammalian pathogens, functions of the O‐polysaccharide (OPS) of lipopolysaccharide (LPS) remain unclear in plant‐associated bacteria. Genetic information about OPS biosynthesis in these bacteria is largely missing. Genome analysis of various plant‐associated Pseudomonas strains revealed that one of the two known OPS biosynthesis clusters from Pseudomonas aeruginosa PAO1, the common polysaccharide antigen (CPA) gene cluster, is only conserved in some strains of the Pseudomonas fluorescens group. For the O‐specific antigen (OSA) biosynthesis cluster, the putative genomic position could be identified, but orthologues of most functional important OSA biosynthesis enzymes could not be detected. Nevertheless, orthologues of the glycosyltransferase WbpL, required for initiation of CPA and OSA synthesis in P. aeruginosa PAO1, could be identified in the analysed Pseudomonas genomes. Knockout mutations of wbpL orthologues in Pseudomonas syringae pv. tomato DC3000 (Pst) and Pseudomonas cichorii ATCC10857/DSM50259 (Pci) resulted in strains lacking the OPS. Infection experiments of Arabidopsis thaliana plants revealed a reduced entry into the leaf apoplast after spray inoculation and a reduced apoplastic amplification of Pst ∆wbpL. Stab and spray inoculation of lettuce (Lactuca sativa) leaves with Pci ∆wbpL causes reduced infection symptoms compared to the wild‐type strain. Furthermore, swarming motility was reduced in ∆wbpL mutants of Pst and Pci. This might be a possible reason for reduced bacterial titres after surface inoculation and reduced bacterial amplification in the plant. Our results imply that the presence of lipopolysaccharide OPS is required for efficient host colonization and full virulence of plant‐pathogenic Pseudomonas bacteria. |
| اللغة: | English |
| تدمد: | 1364-3703 1464-6722 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=pmid________::5d2b85b3e467e3e7a50f9653e0b8ffdaTest http://europepmc.org/articles/PMC6804347Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.pmid..........5d2b85b3e467e3e7a50f9653e0b8ffda |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13643703 14646722 |
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