Sequencing of bulks of segregants allows dissection of genetic control of amylose content in rice
| العنوان: | Sequencing of bulks of segregants allows dissection of genetic control of amylose content in rice |
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| المؤلفون: | Agnelo Furtado, Marie-Noelle Ndjiondjop, Robert J Henry, Peterson W. Wambugu |
| المصدر: | Plant Biotechnology Journal |
| بيانات النشر: | John Wiley and Sons Inc., 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, bulk segregant analysis, Genetic Linkage, Locus (genetics), Plant Science, Biology, Oryza glaberrima, 01 natural sciences, Polymorphism, Single Nucleotide, 03 medical and health sciences, Gene Frequency, Genetic linkage, Gene Expression Regulation, Plant, Gene, Research Articles, Plant Proteins, Genetics, Whole genome sequencing, whole genome sequencing, Oryza sativa, Chromosome, food and beverages, Oryza, biology.organism_classification, 030104 developmental biology, Genetic marker, amylose content, genetic markers, Amylose, Agronomy and Crop Science, allele frequency, 010606 plant biology & botany, Biotechnology, Research Article |
| الوصف: | Summary Amylose content (AC) is a key quality trait in rice. A cross between Oryza glaberrima (African rice) and Oryza sativa (Asian rice) segregating for AC was analysed by sequencing bulks of individuals with high and low AC. SNP associated with the granule bound starch synthase (GBSS1) locus on chromosome 6 were polymorphic between the bulks. In particular, a G/A SNP that would result in an Asp to Asn mutation was identified. This amino acid substitution may be responsible for differences in GBSS activity as it is adjacent to a disulphide linkage conserved in all grass GBSS proteins. Other polymorphisms in genomic regions closely surrounding this variation may be the result of linkage drag. In addition to the variant in the starch biosynthesis gene, SNP on chromosomes 1 and 11 linked to AC was also identified. SNP was found in the genes encoding the NAC and CCAAT‐HAP5 transcription factors that have previously been linked to starch biosynthesis. This study has demonstrated that the approach of sequencing bulks was able to identify genes on different chromosomes associated with this complex trait. |
| اللغة: | English |
| تدمد: | 1467-7652 1467-7644 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66bd81ad07538ce5f8230a58278a9609Test http://europepmc.org/articles/PMC5785344Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....66bd81ad07538ce5f8230a58278a9609 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14677652 14677644 |
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