Age attenuates the transcriptional changes that occur with sleep in the medial prefrontal cortex
| العنوان: | Age attenuates the transcriptional changes that occur with sleep in the medial prefrontal cortex |
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| المؤلفون: | Xiaofeng Guo, Brendan T. Keenan, Diane C. Lim, Gregory R. Grant, Allan I. Pack, Dimitra Sarantopoulou, Jie Lian |
| المصدر: | Aging Cell |
| بيانات النشر: | John Wiley and Sons Inc., 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, medicine.medical_specialty, Aging, Synaptogenesis, Prefrontal Cortex, Biology, Transcriptome, next‐generation RNA sequencing, 03 medical and health sciences, Mice, 0302 clinical medicine, Internal medicine, medicine, Animals, sleep, Prefrontal cortex, Arc (protein), functions of sleep, Cell Biology, Original Articles, Sleep in non-human animals, sleep deprivation, Oligodendrocyte, Mice, Inbred C57BL, Sleep deprivation, 030104 developmental biology, Endocrinology, medicine.anatomical_structure, Wakefulness, Original Article, medicine.symptom, 030217 neurology & neurosurgery, medial prefrontal cortex |
| الوصف: | Sleep abnormalities are common with aging. Studies show that sleep plays important roles in brain functions, and loss of sleep is associated with increased risks for neurological diseases. Here, we used RNA sequencing to explore effects of age on transcriptome changes between sleep and sleep deprivation (SD) in medial prefrontal cortex and found that transcriptional changes with sleep are attenuated in old. In particular, old mice showed a 30% reduction in the number of genes significantly altered between sleep/wake and, in general, had smaller magnitudes of changes in differentially expressed genes compared to young mice. Gene ontology analysis revealed differential age effects on certain pathways. Compared to young mice, many of the wake‐active functions were similarly induced by SD in old mice, whereas many of the sleep‐active pathways were attenuated in old mice. We found similar magnitude of changes in synaptic homeostasis genes (Fos, Arc, and Bdnf) induced by SD, suggesting intact synaptic upscaling on the transcript level during extended wakefulness with aging. However, sleep‐activated processes, such as DNA repair, synaptogenesis, and axon guidance, were sensitive to the effect of aging. Old mice expressed elevated levels of immune response genes when compared to young mice, and enrichment analysis using cell‐type‐specific markers indicated upregulation of microglia and oligodendrocyte genes in old mice. Moreover, gene sets of the two cell types showed age‐specific sleep/wake regulation. Ultimately, this study enhances understanding of the transcriptional changes with sleep and aging, providing potential molecular targets for future studies of age‐related sleep abnormalities and neurological disorders. |
| اللغة: | English |
| تدمد: | 1474-9726 1474-9718 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c98e453f52221af1ed898c696c5863bTest http://europepmc.org/articles/PMC6826131Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8c98e453f52221af1ed898c696c5863b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14749726 14749718 |
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